概述

  • 产品名称Anti-Histone H2B (acetyl K12)抗体
    参阅全部 Histone H2B 一抗
  • 描述
    兔多克隆抗体to Histone H2B (acetyl K12)
  • 特异性ab61228 detects endogenous levels of Histone H2B only when acetylated at lysine 12. (Human: Lys12; Mouse: Lys12; Rat: Lys12)
  • 经测试应用适用于: WB, IHC-P, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide within Human Histone H2B (acetyl K12). The exact sequence is proprietary.
    Sequence:

    PKK(Ac)GS

  • 阳性对照
    • Human lung carcinoma tissue (IHC-P). HeLa cells (ICC/IF). COS7 cells treated with TSA (400nM, 24hours) (WB).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab61228 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab61228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/5000.
ICC/IF Use at an assay dependent concentration.

靶标

Anti-Histone H2B (acetyl K12) antibody 图像

  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Histone H2B (acetyl K12) antibody (ab61228) at 1/50 dilution, in the presence (right panel) or absence (left panel) of acetylated peptide.
  • All lanes : Anti-Histone H2B (acetyl K12) antibody (ab61228) at 1/500 dilution

    Lane 1 : COS7 cells treated with TSA (400nM, 24hours), without acetylated peptide
    Lane 2 : COS7 cells treated with TSA (400nM, 24hours) with acetylated peptide


    Predicted band size : 14 kDa
    Observed band size : 14 kDa

Anti-Histone H2B (acetyl K12) antibody (ab61228)参考文献

This product has been referenced in:
  • Hait NC  et al. Active, phosphorylated fingolimod inhibits histone deacetylases and facilitates fear extinction memory. Nat Neurosci 17:971-80 (2014). Mouse . Read more (PubMed: 24859201) »

See 1 Publication for this product

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