Anti-Histone H2A.Z抗体[EPR6172] (ab124793)


  • 产品名称Anti-Histone H2A.Z抗体[EPR6172]
    参阅全部 Histone H2A.Z 一抗
  • 描述
    兔单克隆抗体[EPR6172] to Histone H2A.Z
  • 特异性This antibody is known to detect both isoforms of H2A.Z (H2A.Z.1 and H2A.Z.2).
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC/IF or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Histone H2A.Z aa 100 to the C-terminus (C terminal).

  • 阳性对照
    • C6, Raw 264.7, PC-12, NIH/3T3, HeLa, HepG2, and Human thymus lysates , Human breast tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EPR6172
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab124793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IHC-P 1/250 - 1/500. antigen retrieval is recommended.
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF or IP.
  • 靶标

    • 功能Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
    • 序列相似性Belongs to the histone H2A family.
    • 翻译后修饰Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
      Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
      Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
      Not phosphorylated.
    • 细胞定位Nucleus. Chromosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • H2A histone family member Z antibody
      • H2A.z antibody
      • H2A/z antibody
      • H2afz antibody
      • H2AZ antibody
      • H2AZ_HUMAN antibody
      • Histone H2A.Z antibody
      • MGC117173 antibody
      see all

    Anti-Histone H2A.Z antibody [EPR6172] 图像

    • All lanes : Anti-Histone H2A.Z antibody [EPR6172] (ab124793) at 1/1000 dilution

      Lane 1 : C6 lysates
      Lane 2 : Raw 264.7 lysates
      Lane 3 : PC-12 lysates
      Lane 4 : NIH/3T3 lysates
      Lane 5 : HeLa lysates
      Lane 6 : HepG2 lysates
      Lane 7 : Human thymus lysates

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 14 kDa
    • ab124793, at a 1/250 dilution, staining Histone H2A.Z in paraffin embedded Human breast tissue by Immunohistochemistry.

    Anti-Histone H2A.Z antibody [EPR6172] (ab124793)参考文献

    ab124793 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 3000 cells
    Gel Running Conditions Reduced Denaturing (15%)
    Sample Human Cell lysate - nuclear (Melanoma)
    Specification Melanoma
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

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    提交于 Sep 01 2014