重组
RabMAb

Anti-Histone H2A.Z抗体[EPR6172] (ab124793)

概述

  • 产品名称
    Anti-Histone H2A.Z抗体[EPR6172]
    参阅全部 Histone H2A.Z 一抗
  • 描述
    兔单克隆抗体[EPR6172] to Histone H2A.Z
  • 特异性
    This antibody is known to detect both isoforms of H2A.Z (H2A.Z.1 and H2A.Z.2).
  • 经测试应用
    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC/IF or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Histone H2A.Z aa 100 to the C-terminus (C terminal).

  • 阳性对照
    • C6, Raw 264.7, PC-12, NIH/3T3, HeLa, HepG2, and Human thymus lysates , Human breast tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    EPR6172
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab124793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IHC-P 1/250 - 1/500. antigen retrieval is recommended.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • 靶标

    • 功能
      Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
    • 序列相似性
      Belongs to the histone H2A family.
    • 翻译后修饰
      Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
      Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
      Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
      Not phosphorylated.
    • 细胞定位
      Nucleus. Chromosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • H2A histone family member Z antibody
      • H2A.z antibody
      • H2A/z antibody
      • H2afz antibody
      • H2AZ antibody
      • H2AZ_HUMAN antibody
      • Histone H2A.Z antibody
      • MGC117173 antibody
      see all

    图片

    • All lanes : Anti-Histone H2A.Z antibody [EPR6172] (ab124793) at 1/1000 dilution

      Lane 1 : C6 lysates
      Lane 2 : Raw 264.7 lysates
      Lane 3 : PC-12 lysates
      Lane 4 : NIH/3T3 lysates
      Lane 5 : HeLa lysates
      Lane 6 : HepG2 lysates
      Lane 7 : Human thymus lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 14 kDa
    • ab124793, at a 1/250 dilution, staining Histone H2A.Z in paraffin embedded Human breast tissue by Immunohistochemistry.

    文献

    ab124793 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Application
    Western blot
    Loading amount
    3000 cells
    Gel Running Conditions
    Reduced Denaturing (15%)
    Sample
    Human Cell lysate - nuclear (Melanoma)
    Specification
    Melanoma
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

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    Verified customer

    提交于 Sep 01 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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