Anti-Histone H2A.Z抗体- ChIP Grade (ab4626)

概述

  • 产品名称
    Anti-Histone H2A.Z抗体- ChIP Grade
    参阅全部 Histone H2A.Z 一抗
  • 描述
    兔多克隆抗体to Histone H2A.Z - ChIP Grade
  • 经测试应用
    适用于: ChIP, WB, IPmore details
  • 种属反应性
    与反应: Saccharomyces cerevisiae
  • 免疫原

    Synthetic peptide corresponding to Saccharomyces cerevisiae Histone H2A.Z aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q12692
    (Peptide available as ab17826)

  • 阳性对照
    • This antibody gave a positive signal in S.cerevisiae (Y190) Whole Cell Lysate

性能

应用

Our Abpromise guarantee covers the use of ab4626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
IP Use at an assay dependent dilution. PubMed: 17373856

靶标

  • 功能
    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
  • 序列相似性
    Belongs to the histone H2A family.
  • 翻译后修饰
    Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
    Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
    Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
    Not phosphorylated.
  • 细胞定位
    Nucleus. Chromosome.
  • Information by UniProt
  • 别名
    • H2A histone family member Z antibody
    • H2A.z antibody
    • H2A/z antibody
    • H2afz antibody
    • H2AZ antibody
    • H2AZ_HUMAN antibody
    • Histone H2A.Z antibody
    • MGC117173 antibody
    see all

图片

  • Anti-Histone H2A.Z antibody - ChIP Grade (ab4626) at 1 µg/ml + S.cerevisiae (Y190) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 14 kDa
    Observed band size : 14 kDa


    Exposure time : 20 minutesAbcam recommends using 3% milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • ab4626 used in ChIP.
    Nuclear lysate prepared from S cerevisiae S288c was used.
    Cross linking (X-ChIP) was performed for 15 seconds with 1% formaldehyde.
    The detection step was performed using Real-time PCR.
    ab4626 was used at a 1/100 dilution for 4 hours at 4°C.

    See Abreview

文献

This product has been referenced in:

See all 15 Publications for this product

客户评价及客户问答


I have aligned both protein sequences - the overall homology is 77%, but for the immunogen region (N-term.) there is unfortunately no homology. Thus, this antibody would not work with S.pombe.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Detection step
Real-time PCR
Sample
Saccharomyces cerevisiae Cell lysate - nuclear (S288c)
Specification
S288c
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Fromaldehyde
Username

Mr. Harsh Ja

Verified customer

提交于 Aug 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (15% gel)
Sample
Saccharomyces cerevisiae Cell lysate - nuclear (S288c)
Specification
S288c
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Mr. Harsh Ja

Verified customer

提交于 Aug 09 2011

Application
ChIP on chip
Detection step
Microarray
Sample
Saccharomyces cerevisiae Cell lysate - nuclear (BY4741 background)
Specification
BY4741 background
Negative control
No antibody control
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 20 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Username

Abcam user community

Verified customer

提交于 May 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Detection step
Semiquantitative PCR
Sample
Saccharomyces cerevisiae Cell lysate - nuclear (BY4741 background)
Specification
BY4741 background
Negative control
No antibody control
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Positive control
Htz1 deletion
Username

Abcam user community

Verified customer

提交于 May 31 2011

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (15% gel)
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (Yeast BY4741 background)
Specification
Yeast BY4741 background
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 May 26 2011

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (S288c background)
Specification
S288c background
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Aug 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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