Anti-Histone H2A.Z抗体- ChIP Grade (ab18263)

概述

  • 产品名称Anti-Histone H2A.Z抗体- ChIP Grade
    参阅全部 Histone H2A.Z 一抗
  • 描述
    羊多克隆抗体to Histone H2A.Z - ChIP Grade
  • 经测试应用适用于: ICC/IF, ChIP, WBmore details
  • 种属反应性
    与反应: Mouse, Chicken, Human, Drosophila melanogaster
  • 免疫原

    Synthetic peptide corresponding to Chicken Histone H2A.Z aa 1-17 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    AGGKAGKDSGKAKAKA

性能

应用

Our Abpromise guarantee covers the use of ab18263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
ChIP Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).

靶标

  • 功能Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
  • 序列相似性Belongs to the histone H2A family.
  • 翻译后修饰Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
    Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
    Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
    Not phosphorylated.
  • 细胞定位Nucleus. Chromosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • H2A histone family member Z antibody
    • H2A.z antibody
    • H2A/z antibody
    • H2afz antibody
    • H2AZ antibody
    • H2AZ_HUMAN antibody
    • Histone H2A.Z antibody
    • MGC117173 antibody
    see all

Anti-Histone H2A.Z antibody - ChIP Grade 图像

  • All lanes : Anti-Histone H2A.Z antibody - ChIP Grade (ab18263)

    Lane 1 : Butyrate-treated HeLa histones
    Lane 2 : 15-day embryo chicken erythrocyte histones
    Lane 3 : Recombinant H2A.Z
    Lane 4 : Butyrate-treated HeLa histones
    Lane 5 : 15-day embryo chicken erythrocyte histones
    Lane 6 : Recombinant H2A.Z


    Predicted band size : 13 kDa
    Observed band size : 13 kDa

    This image is courtesy of Prof Colyn Crane-Robinson

  • All lanes : Anti-Histone H2A.Z antibody - ChIP Grade (ab18263)

    Lane 1 : Butyrate-treated HeLa histones
    Lane 2 : Ix3 extract
    Lane 3 : Nucleosomes from 10-day chicken brain tissue. ChIP Input.
    Lane 4 : Nucleosomes from 10-day chicken brain tissue. ChIP Unbound.
    Lane 5 : Nucleosomes from 10-day chicken brain tissue. ChIP Bound.
    Lane 6 : Butyrate-treated HeLa histones
    Lane 7 : Ix3 extract
    Lane 8 : Nucleosomes from 10-day chicken brain tissue. ChIP Input.
    Lane 9 : Nucleosomes from 10-day chicken brain tissue. ChIP Unbound.
    Lane 10 : Nucleosomes from 10-day chicken brain tissue. ChIP Bound.


    Predicted band size : 13 kDa
    Observed band size : 13 kDa

    This image is courtesy of Prof Colyn Crane-Robinson

    Nucleosomes from 10-day chicken brain tissue were used in ChIP experiments. An acetic acid / urea / Triton gel was run and the corresponding western blot using ab18263 was used to compare proteins in the Bound chromatin fraction with those of the Input and Unbound fractions. In lane 10 there is a strong band corresponding to a hyper-acetylated H2A.Z.

Anti-Histone H2A.Z antibody - ChIP Grade (ab18263)参考文献

This product has been referenced in:

See all 7 Publications for this product

Product Wall

The amino acid sequence of the immunogen that was used to generate the Anti-Histone H2A.Z antibody ab18263 is 100% conserved in the sequence you sent. We expect the antibody will cross-react with Strongylocentrotus purpuratus but we do not guarantee th...

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Thank you for contacting us. Unfortunately, these H2A.Z- and H2A antibodies have not been tested in ELISA or dot blots and we therefore cannot guarantee them to work in these applications. Ab18262 and ab18263 have been tested in Western blot aga...

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Thank you for your enquiry. I have performed an alignment with the sequences of each of the immunogens of ab4174 and ab18263 against Drosophila sequence NP_524519. Once again the results can be viewed below. http://www.ebi.ac.uk/cgi-bin/clustalw...

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Thank you for your enquiry. US Customs are currently preventing all antibodies raised in sheep, goat, donkey, horse, pig and chicken from entering into the States unless you have a USDA import permit in place for these items. You can view inform...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"