Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H2A.Z antibody [4A4]

Average
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreatic cancer)
Specification
Pancreatic cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Other product details

Dilution
1/1000
Incubation time
45 minute(s) · Temperature: 20°C · Diluent: Antibody Diluent (Dako)

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit/mouse HRP polymer (pre-diluted, Dako)
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Additional data

Additional Notes
After the primary antibody incubation a rabbit anti-rat link (Dako) was used (1/400 for 30min at 20oC) before the anti-rabbit polymer HRP was applied.
Three H2A.Z antibodies were tested in parallel (AB80150, AB80153 and AB4462). The staining pattern was very similar in each case and all three could probably be used up to 1/5000 dilution in order to reduce background. Other possible suggestions would be a longer antigen retrieval and an overnight primary incubation at 4oC.
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Mr. Antibody Solutions

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提交于 Mar 11 2010

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