Anti-Histone H2A.X抗体(ab117750)


  • 产品名称Anti-Histone H2A.X抗体
    参阅全部 Histone H2A.X 一抗
  • 描述
    兔多克隆抗体to Histone H2A.X
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    This information is considered to be commercially sensitive.



Our Abpromise guarantee covers the use of ab117750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/25000. Predicted molecular weight: 15 kDa.
ICC/IF 1/400 - 1/800.


  • 功能Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
  • 序列相似性Belongs to the histone H2A family.
  • 发展阶段Synthesized in G1 as well as in S-phase.
  • 结构域The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
  • 翻译后修饰Phosphorylated on Ser-140 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Phosphorylation of Ser-140 (H2AX139ph) in response to ionizing radiation is mediated by both ATM and PRKDC while defects in DNA replication induce Ser-140 phosphorylation (H2AX139ph) subsequent to activation of ATR and PRKDC. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. In meiosis, Ser-140 phosphorylation (H2AX139ph) may occur at synaptonemal complexes during leptotene as an ATM-dependent response to the formation of programmed DSBs by SPO11. Ser-140 phosphorylation (H2AX139ph) may subsequently occurs at unsynapsed regions of both autosomes and the XY bivalent during zygotene, downstream of ATR and BRCA1 activation. Ser-140 phosphorylation (H2AX139ph) may also be required for transcriptional repression of unsynapsed chromatin and meiotic sex chromosome inactivation (MSCI), whereby the X and Y chromosomes condense in pachytene to form the heterochromatic XY-body. During immunoglobulin class switch recombination in lymphocytes, Ser-140 phosphorylation (H2AX139ph) may occur at sites of DNA-recombination subsequent to activation of the activation-induced cytidine deaminase AICDA. Phosphorylation at Tyr-143 (H2AXY142ph) by BAZ1B/WSTF determines the relative recruitment of either DNA repair or pro-apoptotic factors. Phosphorylation at Tyr-143 (H2AXY142ph) favors the recruitment of APBB1/FE65 and pro-apoptosis factors such as MAPK8/JNK1, triggering apoptosis. In contrast, dephosphorylation of Tyr-143 by EYA proteins (EYA1, EYA2, EYA3 or EYA4) favors the recruitment of MDC1-containing DNA repair complexes to the tail of phosphorylated Ser-140 (H2AX139ph).
    Monoubiquitination of Lys-120 (H2AXK119ub) by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
  • 细胞定位Nucleus. Chromosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AW228881 antibody
    • H2A histone family member X antibody
    • H2A.FX antibody
    • H2A.X antibody
    • H2a/x antibody
    • H2AFX antibody
    • H2AX antibody
    • H2AX histone antibody
    • H2AX_HUMAN antibody
    • Hist5.2ax antibody
    • Histone 2A antibody
    • Histone 2AX antibody
    • Histone H2A.X antibody
    • Histone H2AX antibody
    • RGD1566119 antibody
    see all

Anti-Histone H2A.X antibody 图像

  • Lanes 1 - 3 : Anti-Histone H2A.X antibody (ab117750) at 0.2 µg/ml
    Lanes 4 - 6 : Anti-Histone H2A.X antibody (ab117750) at 0.1 µg/ml
    Lanes 7 - 9 : Anti-Histone H2A.X antibody (ab117750) at 0.04 µg/ml

    Lane 1 : Nuclear extract from wild-type mouse embryonic fibroblasts
    Lane 2 : Nuclear extract from H2AX knockout mouse embryonic fibroblasts
    Lane 3 : Nuclear extract from Human 293T cells
    Lane 4 : Nuclear extract from wild-type mouse embryonic fibroblasts
    Lane 5 : Nuclear extract from H2AX knockout mouse embryonic fibroblasts
    Lane 6 : Nuclear extract from Human 293T cells
    Lane 7 : Nuclear extract from wild-type mouse embryonic fibroblasts
    Lane 8 : Nuclear extract from H2AX knockout mouse embryonic fibroblasts
    Lane 9 : Nuclear extract from Human 293T cells

    Predicted band size : 15 kDa
    Observed band size : 15 kDa

Anti-Histone H2A.X antibody (ab117750)参考文献

ab117750 has not yet been referenced specifically in any publications.

Product Wall

I can confirm that ab11175 will only bind H2AX that is not phosphorylated on the Ser 139 i.e. it does not appear to bind to gamma H2AX. Blast search shows that it should not cross-react with H2A. If you require an antibody that will detect the ...

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