Anti-Histone H2A (phospho S129)抗体(ab17353)
Key features and details
- Rabbit polyclonal to Histone H2A (phospho S129)
- Suitable for: WB
- Reacts with: Schizosaccharomyces pombe
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Histone H2A (phospho S129)抗体
参阅全部 Histone H2A 一抗 -
描述
兔多克隆抗体to Histone H2A (phospho S129) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Schizosaccharomyces pombe
预测可用于: Saccharomyces cerevisiae -
免疫原
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阳性对照
- S. pombe lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab17353于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 15.5 kDa (predicted molecular weight: 15.5 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 15.5 kDa (predicted molecular weight: 15.5 kDa). |
靶标
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功能
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
序列相似性
Belongs to the histone H2A family. -
翻译后修饰
The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
Deiminated on Arg-4 in granulocytes upon calcium entry.
Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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数据库链接
- SwissProt: P04912 Saccharomyces cerevisiae
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别名
- H2a 615 antibody
- H2A antibody
- H2A GL101 antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab17353 被引用在 9 文献中.
- Schmücker A et al. Crosstalk between H2A variant-specific modifications impacts vital cell functions. PLoS Genet 17:e1009601 (2021). PubMed: 34086674
- Tatebe H et al. Rad50 zinc hook functions as a constitutive dimerization module interchangeable with SMC hinge. Nat Commun 11:370 (2020). PubMed: 31953386
- Cal M et al. The Anticancer Drug 3-Bromopyruvate Induces DNA Damage Potentially Through Reactive Oxygen Species in Yeast and in Human Cancer Cells. Cells 9:N/A (2020). PubMed: 32397119
- Kim SM & Forsburg SL Active Replication Checkpoint Drives Genome Instability in Fission Yeast mcm4 Mutant. Mol Cell Biol 40:N/A (2020). PubMed: 32341083
- Jablonowski CM et al. Termination of Replication Stress Signaling via Concerted Action of the Slx4 Scaffold and the PP4 Phosphatase. Genetics 201:937-49 (2015). PubMed: 26362319
- Tapia-Alveal C et al. H2A.Z-dependent regulation of cohesin dynamics on chromosome arms. Mol Cell Biol 34:2092-104 (2014). PubMed: 24687850
- Lee M et al. Rad52/Rad59-dependent recombination as a means to rectify faulty Okazaki fragment processing. J Biol Chem 289:15064-79 (2014). PubMed: 24711454
- Garcia V et al. Bidirectional resection of DNA double-strand breaks by Mre11 and Exo1. Nature 479:241-4 (2011). PubMed: 22002605
- Outwin EA et al. Smc5-Smc6-dependent removal of cohesin from mitotic chromosomes. Mol Cell Biol 29:4363-75 (2009). WB ; Schizosaccharomyces pombe . PubMed: 19528228