Anti-Histone H2A (di methyl K95)抗体(ab4633)

概述

  • 产品名称Anti-Histone H2A (di methyl K95)抗体
    参阅全部 Histone H2A 一抗
  • 描述
    兔多克隆抗体to Histone H2A (di methyl K95)
  • 特异性In ELISA analysis ab4633 recognises di methyl histone H2A K95 but not the corresponding unmodified or histone H4 di methyl K79 peptides. However, ab4633 does to an extent also recognise a di methyl K99 peptide in ELISA analysis.
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Chicken, Cow, Human
    预测可用于: Mouse, Rat, Duck, a wide range of other species
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Histone H2A, di methylated at K95.

    (Peptide available as ab10127.)

  • 阳性对照
    • Calf Thymus Histone Preparation

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab4633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000. Detects a band of approximately 15 kDa.

靶标

  • 功能Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 序列相似性Belongs to the histone H2A family.
  • 翻译后修饰The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
    Deiminated on Arg-4 in granulocytes upon calcium entry.
    Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
    Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
    Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
  • 细胞定位Nucleus. Chromosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FO108 antibody
    • H2A antibody
    • H2A1B_HUMAN antibody
    • H4 histone family 2 antibody
    • H4 histone family member N antibody
    • H4F2 antibody
    • H4FN antibody
    • HIST1H2AE antibody
    • HistH2A antibody
    • Histone H2A type 1-B/E antibody
    • Histone H2A.2 antibody
    • Histone H2A/a antibody
    • Histone H2A/m antibody
    see all

Anti-Histone H2A (di methyl K95) antibody 图像

  • ICC/IF image of ab4633 stained human HEK 293 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab4633, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • Lanes 1 & 3 : Anti-Histone H2A (di methyl K95) antibody (ab4633) at 1/500 dilution
    Lanes 2 & 4 : Anti-Histone H2A (di methyl K95) antibody (ab4633) at 1/1000 dilution

    Lane 1 : 1ug of calf thymus Histone prep
    Lane 2 : 1ug of calf thymus Histone prep
    Lane 3 : 1ug of calf thymus Histone prep with Human Histone H2A (di methyl K95) peptide (ab10127) at 2 µg
    Lane 4 : 1ug of calf thymus Histone prep with Human Histone H2A (di methyl K95) peptide (ab10127) at 2 µg

    Secondary
    Lanes 1 - 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 2000
    Lane 4 : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

Anti-Histone H2A (di methyl K95) antibody (ab4633)参考文献

ab4633 has not yet been referenced specifically in any publications.

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