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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H2A, asymmetric di methylated at R3.
(Peptide available as ab22398.)
Our Abpromise guarantee covers the use of ab21574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration.
This antibody gave a positive result in ELISA against the immunizing peptide (ab22398). For the following peptides it gave following results: the non-modified equivalent peptide (ab13186) - negative result, Histone H2A (symmetric di methyl R3)peptide(ab22399) - negative result. This indicates that it is specific for the Histone H2A (asymmetric di methyl R3) peptide. See figure below. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
IHC image of ab21574 staining Histone H2A (asymmetric di methyl R3) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21574, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This antibody gave a positive result in ELISA against the immunizing peptide ab22398 (blue line).
For the following peptides it gave following results:
Histone H2A (symmetric di methyl R3) peptide ab22399 (pink line) - negative result,
the non-modified equivalent peptide ab13186 (yellow line) - negative result.
This indicates that it is specific for the Histone H2A (asymmetric di methyl R3) peptide.
ab21574 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"