Anti-Histone H2A抗体- ChIP Grade (ab13923)


  • 产品名称Anti-Histone H2A抗体- ChIP Grade
    参阅全部 Histone H2A 一抗
  • 描述
    兔多克隆抗体to Histone H2A - ChIP Grade
  • 经测试应用适用于: IHC-P, ELISA, WB, ChIPmore details
  • 种属反应性
    与反应: Human, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe
    预测可用于: Mouse, Rat, Chicken, Saccharomyces cerevisiae, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Human Histone H2A aa 1-15. This antibody was generated by immunizing rabbits with a mixture of synthetic peptides containing amino acid residues 1-15 (MSGRGKQGGKARAKA) and 81-96 (PRHLQLAIRNDEELNK) of human Histone H2A.


  • 阳性对照
    • Human peripheral blood cell lysates or Jurkat cell lysate.



Our Abpromise guarantee covers the use of ab13923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
ELISA 1/1000.

See Dai et al, 2008 for details on Nucleosome ELISA

WB Use a concentration of 2 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 16 kDa).
ChIP Use at an assay dependent concentration. PubMed: 20711347


  • 功能Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 序列相似性Belongs to the histone H2A family.
  • 翻译后修饰The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
    Deiminated on Arg-4 in granulocytes upon calcium entry.
    Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
    Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
    Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
  • 细胞定位Nucleus. Chromosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • H2a 615 antibody
    • H2A antibody
    • H2A GL101 antibody
    • H2A histone family member A antibody
    • H2A.1 antibody
    • H2A.2 antibody
    • H2A/a antibody
    • H2A/m antibody
    • H2A/O antibody
    • H2A/q antibody
    • H2A1B_HUMAN antibody
    • H2AFA antibody
    • H2AFE antibody
    • H2AFL antibody
    • H2AFM antibody
    • H2AFO antibody
    • H2AFQ antibody
    • HIST1H2AE antibody
    • HIST1H2AJ antibody
    • HIST2H2AA antibody
    • HIST2H2AA3 antibody
    • HIST2H2AB antibody
    • HIST2H2AC antibody
    • Histone 1 H2ae antibody
    • Histone 2 H2aa3 antibody
    • Histone 2 H2ab antibody
    • Histone 2 H2ac antibody
    • Histone H2A type 1 B antibody
    • Histone H2A type 1 C antibody
    • Histone H2A type 1 E antibody
    • Histone H2A type 1 J antibody
    • Histone H2A type 1-B/E antibody
    • Histone H2A.2 antibody
    • Histone H2A/a antibody
    • Histone H2A/m antibody
    • MGC74460 antibody
    see all

Anti-Histone H2A antibody - ChIP Grade 图像

  • ICC/IF image of ab13923 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13923, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Predicted band size : 16 kDa

    Western blot analysis of Histone H2A in human PBMC cell lysate with ab13923.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast tissue (ab30090)labelling Histone H2A with ab13923 at 5µg/ml. Staining was enhanced by boiling tissue sections in 10mM sodium citrate buffer, pH6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

  • ab13923 staining Human normal skin (ab30166). Staining is localised to nuclear compartment.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Histone H2A antibody - ChIP Grade (ab13923)参考文献

This product has been referenced in:

See all 20 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - nuclear (MEF)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification MEF
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Apr 06 2017

Thank you for your reply.

I am very sorry for the confusion. I have checked back in the records and I made a mistake in looking through the correspondence. I am very sorry for this.

I am sorry that the suggested optimisations have not...

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Thank you very much for your reply.

The reason why I believe it would be worth trying to optimise further is because in the 3 different blots you shared with me there is a non-specific band at ˜25 kDa. This suggests that there may be something...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with the Anti-Histone H2A antibody (ab13923). We take product complaints very seriously, and investigate every product that we feel may not be performing correct...

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Thank you for contacting Abcam.

I am sorry that ab13923 did not work for you in Xenopus however the reason we issued you the discount code is because we could not guarantee that the antibody would work in Xenopus tissue.

Once you su...

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Expiration date: May 18th, 2012

I am very pleased to hear you would like to accept our offer and test ab13923 in xenopus. This code will give you 1 free primary antibody before the expiration date. To redeem this offe...

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Thank you for contacting Abcam regarding this issue. I have had a chance to look at each of the antibodies to Histone H2A in our catalog and compare the immunogen sequences with your specific needs. Unfortunately none of these products fall exclusiv...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human Caco-2 epithelial colorectal adenocarcinoma)
Loading amount 10 µg
Specification Human Caco-2 epithelial colorectal adenocarcinoma
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

提交于 Dec 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Saccharomyces cerevisiae Cell lysate - whole cell (Whole cell extract)
Loading amount 5e+006 cells
Specification Whole cell extract
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Odyssey blocking buffer as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C

Abcam user community

Verified customer

提交于 Jan 20 2010

Thank you for your questions about ab13923. We have not tested cross-reactivity of this antibody to H2AZ. I recommend that you should use the immunogen information previously supplied: PRHLQLAIRNDEELNK 81-96 MSGRGKQGGKARAKA 1-15 The higher ...

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