Anti-Histone H2A (acetyl K5)抗体- ChIP Grade (ab1764)

概述

  • 产品名称Anti-Histone H2A (acetyl K5)抗体- ChIP Grade
    参阅全部 Histone H2A 一抗
  • 描述
    兔多克隆抗体to Histone H2A (acetyl K5) - ChIP Grade
  • 特异性Histone H2A acetylated at lysine 5.
  • 经测试应用适用于: ChIP, ELISA, IP, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human, Caenorhabditis elegans, Drosophila melanogaster, all Mammals, Asian Toad (Bufo)
  • 免疫原

    Synthetic peptide corresponding to Histone H2A (acetyl K5) conjugated to Ovalbumin.
    Sequence:

    SGRGK(Ac)QGGKYC

  • 阳性对照
    • HL60 cells treated with butyrate. IHC-P: Human normal colon FFPE tissue sections.
  • 常规说明

    ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

性能

应用

Our Abpromise guarantee covers the use of ab1764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/800 - 1/2000. Detects a band of approximately 20 kDa.

Use 5% BSA in TBST to block.

IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 序列相似性Belongs to the histone H2A family.
  • 翻译后修饰The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
    Deiminated on Arg-4 in granulocytes upon calcium entry.
    Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
    Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
    Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
  • 细胞定位Nucleus. Chromosome.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • H2A antibody
    • H2A1B_HUMAN antibody
    • H2AFM antibody
    • HIST1H2A antibody
    • HIST1H2AE antibody
    • Histone H2A type 1-B/E antibody
    • Histone H2A.2 antibody
    • Histone H2A/a antibody
    • Histone H2A/m antibody
    see all

Anti-Histone H2A (acetyl K5) antibody - ChIP Grade 图像

  • IHC image of ab1764 staining Histone H2A (acetyl K5) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1764, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC - Wholemount of Caenorhabditis elegans whole body labelling Histone H2A (acetyl K5) with ab1764. Sample was incubated with primary antibody (1/2500 in 3% BSA + 0.1% Triton X-100 in PBS) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Left - Histone H2A (acetyl K5) (green), Right - nuclei (DAPI, blue). 

    See Abreview

  • ab1764 staining Female Embryonic Stem cells differentiated for 7 days by immunocytochemistry. The antibody was used at a dilution of 1/200 and incubated with the cells for 1 hour. Bound antibody was detected with a FITC conjugated Goat anti-Rabbit polyclonal antibody.

    DAPI counterstain is shown pseudocoloured as white on the right of the image (b). Centric heterochromatin was underacetylated in differentiated cells (arrowhead in a). A pale staining chromosome was observed at high frequencies; previoulsy shown to be the inactive female X chromosome (arrow in a).

    This image is courtesy of an Abreview by Hugh Spotswood submitted on 13 February 2006.

    See Abreview



  • Observed band size : 15 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    Ab1764 at a 1/1000 dilution in Western blot of human Osteosarcoma (U2OS) nuclear cell lysate.

    See Abreview

Anti-Histone H2A (acetyl K5) antibody - ChIP Grade (ab1764)参考文献

This product has been referenced in:
  • Michishita E  et al. SIRT6 is a histone H3 lysine 9 deacetylase that modulates telomeric chromatin. Nature : (2008). WB ; Human . Read more (PubMed: 18337721) »
  • Garcia SN  et al. Mrg15 null and heterozygous mouse embryonic fibroblasts exhibit DNA-repair defects post exposure to gamma ionizing radiation. FEBS Lett 581:5275-81 (2007). WB ; Mouse . Read more (PubMed: 17961556) »

See all 5 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (15%)
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Fruit fly embryo)
Specification Fruit fly embryo
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Dec 18 2013

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (15%)
Sample Mouse Cell lysate - nuclear (Mouse embryonic fibroblasts)
Specification Mouse embryonic fibroblasts
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Dec 18 2013

Application IHC - Wholemount
Sample Caenorhabditis elegans Tissue (Adult C. elegans whole body)
Specification Adult C. elegans whole body
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提交于 Dec 18 2013

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (15%)
Sample Caenorhabditis elegans Tissue lysate - whole (C. elegans embryo)
Specification C. elegans embryo
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Dec 18 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Antigen retrieval step Heat mediated
Sample Mouse Tissue sections (Mouse brain)
Specification Mouse brain
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
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提交于 Dec 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (Keratinocytes)
Loading amount 50 µg
Specification Keratinocytes
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Dec 23 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (keratinocytes)
Specification keratinocytes
Fixative Formaldehyde
Permeabilization Yes - 0.1% triton-x-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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提交于 Dec 23 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Embryonic Stem)
Specification Embryonic Stem
Fixative Formaldehyde
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Verified customer

提交于 Apr 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (osteosarcoma (U2OS))
Specification osteosarcoma (U2OS)
Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5%
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Verified customer

提交于 Mar 14 2006

Thank you for your enquiry and for forwarding your completed questionnaire to us. We are very sorry to hear that you are having problem with this antibody. We have looked through your protocol and would like to make some suggestions. 1. First o...

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