This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-Histone H1.4 (tri methyl K25, phospho S26)抗体(ab33231)

概述

  • 产品名称Anti-Histone H1.4 (tri methyl K25, phospho S26)抗体
    参阅全部 Histone H1.4 一抗
  • 描述
    兔多克隆抗体to Histone H1.4 (tri methyl K25, phospho S26)

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • 种属反应性

    预测可用于: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H1.4, tri methylated at K25 and phosphorylated at S26.

    (Peptide available as ab35913.)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

  • 应用说明This antibody gave a positive result in ELISA against the immunizing peptide.
    For the following peptides it gave following results:
    the non-modified equivalent peptide (yellow line, ab35918) - weak result,
    H1.4 phospho S26 peptide (pink line, ab35917) - weak result.
    See figure below.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
    • 序列相似性Belongs to the histone H1/H5 family.
      Contains 1 H15 (linker histone H1/H5 globular) domain.
    • 翻译后修饰Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
    • 细胞定位Nucleus. Chromosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • H1 histone family member 4 antibody
      • H1.4 antibody
      • H14_HUMAN antibody
      • H1E antibody
      • H1F4 antibody
      • Hist1h1e antibody
      • Histone 1 H1e antibody
      • Histone cluster 1 H1e antibody
      • Histone H1 antibody
      • Histone H1.4 antibody
      • Histone H1B antibody
      • MGC116819 antibody
      see all

    Anti-Histone H1.4 (tri methyl K25, phospho S26) antibody 图像

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • This antibody gave a positive result in ELISA against the immunizing peptide (blue line, ab35913).
      For the following peptides it gave following results:
      the non-modified equivalent peptide (yellow line, ab35918) - weak result,
      H1.4 phospho S26 peptide (pink line, ab35917) - weak result.

    Anti-Histone H1.4 (tri methyl K25, phospho S26) antibody (ab33231)参考文献

    ab33231 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Human Purified protein (breast cancer cell line T47D)
    Loading amount 5 µg
    Specification breast cancer cell line T47D
    Treatment 50ng/ml colcemid for 16h
    Gel Running Conditions Non-reduced Denaturing (10 % SDS-PAGE)
    Blocking step Odissey blocking solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: RT°C
    Username

    Dr. Albert Jordan

    Verified customer

    提交于 May 20 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"