Anti-Histone H1.4抗体(ab105522)

概述

  • 产品名称Anti-Histone H1.4抗体
    参阅全部 Histone H1.4 一抗
  • 描述
    兔多克隆抗体to Histone H1.4
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog, African Green Monkey
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MSETAPAAPA APAPAEKTPV KKKARKSAGA AKRKASGPPV SELITKAVAA) of Human Histone H1.4 (NP_005312).

  • 阳性对照
    • ACHN cell lysate IF/ICC: SKNSH cell line.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab105522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa.Can be blocked with Histone H1.4 peptide (ab130902). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
  • 序列相似性Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • 翻译后修饰Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
  • 细胞定位Nucleus. Chromosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dJ221C16.5 antibody
    • H1 histone family, member 4 antibody
    • H1.4 antibody
    • H14_HUMAN antibody
    • H1E antibody
    • H1F4 antibody
    • Hist1h1e antibody
    • Histone 1, H1e antibody
    • Histone cluster 1, H1e antibody
    • Histone H1.4 antibody
    • Histone H1B antibody
    • MGC116819 antibody
    • OTTHUMP00000016139 antibody
    see all

Anti-Histone H1.4 antibody 图像

  • ICC/IF image of ab105522 stained SKNSH cells. The cells were4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105522, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Anti-Histone H1.4 antibody (ab105522) at 1 µg/ml + ACHN cell lysate at 10 µg

    Predicted band size : 22 kDa
    Gel concentration: 12%

Anti-Histone H1.4 antibody (ab105522)参考文献

ab105522 has not yet been referenced specifically in any publications.

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