Anti-Histone H1.11L抗体- ChIP Grade (ab100948)

概述

  • 产品名称Anti-Histone H1.11L抗体- ChIP Grade
  • 描述
    羊多克隆抗体to Histone H1.11L - ChIP Grade
  • 经测试应用适用于: WB, ChIP, IHC-Pmore details
  • 种属反应性
    与反应: Chicken
  • 免疫原

    N-terminal peptide (APAPAAEAAPAAAPAPAK-C) of chicken linker histone H1.llL.

  • 阳性对照
    • IHC-P: Chicken normal colon FFPE tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab100948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.
ChIP Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.

Anti-Histone H1.11L antibody - ChIP Grade 图像

  • IHC image of ab100948 staining Histone H1.11L in chicken colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab100948, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Competition Western Blot Analysis. The recombinant linker histone was separated on a 15% SDS PAGE slab gel together with a track loaded with total chicken histone as marker and transferred to nitrocellulose membranes. These were then cut into strips of equal width and each strip developed with the antibody which had been pre-incubated with one of the recombinant linker histone at four increasing concentrations (two are shown). The panels show (from left to right): the transfer of the recombinant protein slab gel; the transfer of a total chicken histone marker, M; the histone marker strip blotted with the antibody under test, M(WB), showing that there is no recognition of the core histones, nor H5, with the antibody; six strips developed with the test antibody (at 0.1 mg/ml) pre-incubated with the six recombinant histones (01 to R) at the indicated concentrations. The Control lanes represent blots of a seventh strip developed with the test antibody without any competitor. The red boxes

Anti-Histone H1.11L antibody - ChIP Grade (ab100948)参考文献

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