Anti-Histone H1.03抗体- ChIP Grade (ab100946)

概述

  • 产品名称Anti-Histone H1.03抗体- ChIP Grade
  • 描述
    羊多克隆抗体to Histone H1.03 - ChIP Grade
  • 经测试应用适用于: WB, ChIP, IHC-Pmore details
  • 种属反应性
    与反应: Chicken
  • 免疫原

    N-terminal peptide (APVAAPDVAAAPTPAK-C) of chicken linker histone H1.03.

性能

应用

Our Abpromise guarantee covers the use of ab100946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.
ChIP Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.

Anti-Histone H1.03 antibody - ChIP Grade 图像

  • Competition Western Blot Analysis. The recombinant linker histone was separated on a 15% SDS PAGE slab gel together with a track loaded with total chicken histone as marker and transferred to nitrocellulose membranes. These were then cut into strips of equal width and each strip developed with the antibody which had been pre-incubated with one of the recombinant linker histone at four increasing concentrations (two are shown). The panels show (from left to right): the transfer of the recombinant protein slab gel; the transfer of a total chicken histone marker, M; the histone marker strip blotted with the antibody under test, M(WB), showing that there is no recognition of the core histones, nor H5, with the antibody; six strips developed with the test antibody (at 0.1 mg/ml) pre-incubated with the six recombinant histones (01 to R) at the indicated concentrations. The Control lanes represent blots of a seventh strip developed with the test antibody without any competitor. The red boxes show tracks in which signal has been competed away.

Anti-Histone H1.03 antibody - ChIP Grade (ab100946)参考文献

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"