Anti-Histone H1.0抗体(ab83058)

概述

  • 产品名称Anti-Histone H1.0抗体
    参阅全部 Histone H1.0 一抗
  • 描述
    兔多克隆抗体to Histone H1.0
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to Human Histone H1.0 aa 36-85 (N terminal).
    Sequence:

    IQAEKNRAGSSRQSIQKYIKSHYKVGENADSQIKLSIKRLVTTGVLKQTK


    Database link: P07305

  • 阳性对照
    • HeLa cell lysate This antibody gave a positive result in IF in the following Methanol fixed cell line: HeLa.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab83058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
  • 序列相似性Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • 翻译后修饰Phosphorylated on Ser-17 in RNA edited version.
  • 细胞定位Nucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • H1 histone family member 0 antibody
    • H1(0) antibody
    • H10 antibody
    • H10_HUMAN antibody
    • h1f0 antibody
    • H1FV antibody
    • Histone H1'' antibody
    • Histone H1(0) antibody
    • Histone H1.0 antibody
    • Histone H10 antibody
    • Histone H5 antibody
    • MGC5241 antibody
    • N-terminally processed antibody
    see all

Anti-Histone H1.0 antibody 图像

  • Anti-Histone H1.0 antibody (ab83058) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 21 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa,36 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab83058 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab83058 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Histone H1.0 antibody (ab83058)参考文献

ab83058 has not yet been referenced specifically in any publications.

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H1.2 specific antibodies,
ab4086; http://www.abcam.com/Histone-H1-2-antibody-ChIP-Grade-ab4086.html. (we however haven't tested the specificity to other forms)
ab17677; http://www.abcam.com/Histone-H1-2-...

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Thank you for contacting us.

ab87556 was used with a sequence from H1FOO_HUMAN protein; http://www.uniprot.org/uniprot/Q8IZA3. According to swissprot the molecular weight is 35 kDa.

ab83058; the sequence used is from P07305 http://...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"