Anti-Histone H1.0抗体(ab65000)

概述

  • 产品名称
    Anti-Histone H1.0抗体
    参阅全部 Histone H1.0 一抗
  • 描述
    兔多克隆抗体to Histone H1.0
  • 特异性
    Detects endogenous levels of total Histone 1.0 protein
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Histone H1.0 aa 71-120 (internal sequence).
    Database link: P07305

  • 阳性对照
    • A549 cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab65000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ELISA 1/40000.

靶标

  • 功能
    Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
  • 序列相似性
    Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • 翻译后修饰
    Phosphorylated on Ser-17 in RNA edited version.
  • 细胞定位
    Nucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • H1 histone family member 0 antibody
    • H1(0) antibody
    • H10 antibody
    • H10_HUMAN antibody
    • h1f0 antibody
    • H1FV antibody
    • Histone H1'' antibody
    • Histone H1(0) antibody
    • Histone H1.0 antibody
    • Histone H10 antibody
    • Histone H5 antibody
    • MGC5241 antibody
    • N-terminally processed antibody
    see all

Anti-Histone H1.0 antibody 图像

  • All lanes : Anti-Histone H1.0 antibody (ab65000) at 1/500 dilution

    Lane 1 : Extracts from A549 cells at 10-30µg
    Lane 2 : Extracts from A549 cells at 10-30µg with immunising peptide at 5-10µg


    Predicted band size : 21 kDa
    Observed band size : 21 kDa
    Additional bands at : 110 kDa,23 kDa,32 kDa,34 kDa,36 kDa,46 kDa. We are unsure as to the identity of these extra bands.

实验方案

Anti-Histone H1.0 antibody (ab65000)参考文献

ab65000 has not yet been referenced specifically in any publications.

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