Anti-Histone H1 (tri methyl K25)抗体(ab17347)

概述

  • 产品名称Anti-Histone H1 (tri methyl K25)抗体
  • 描述
    兔多克隆抗体to Histone H1 (tri methyl K25)
  • 特异性ab17347 gives a positive result against the immunizing peptide and a negative result against the corresponding unmodified peptide in ELISA analysis. ab17347 recognises histone H1 tri methyl K25 in HeLa nuclear and whole cell lysates at 35 kDa. The signal is efficiently blocked using the immunzing histone H1 tri methyl K25 peptide but not the corrseponding unmodified peptide or histone H1 di methyl K25 peptide. ab17347 also appears to recognise other bands between 34 kDa and 37 kDa, which are attributed to the antibody recognising other isoforms of histone H1. ab17347 also appears to recognise methylated histone H3 at 17 kDa.
  • 经测试应用适用于: IHC-FoFr, WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Cow, Human
    预测可用于: Non Human Primates
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H1, tri methylated at K25.

  • 阳性对照
    • Hela whole cell lysate
  • 常规说明HeLa nuclear lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab17347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr 1/200.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 相关性Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in the formation of higher order structure in chromatin and the maintenance of overall chromatin compaction. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono-, di-, or trimethylated state. Arginine resdiues can also by mono or di methylated.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • H1 antibody
    • H1.4 antibody
    • histone H1.4 antibody

Anti-Histone H1 (tri methyl K25) antibody 图像

  • All lanes : Anti-Histone H1 (tri methyl K25) antibody (ab17347) at 1 µg/ml

    Lane 1 : HeLa nuclear lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : HeLa nuclear lysate with Human Histone H1 (tri methyl K25) peptide (ab17587) at 1 µg/ml
    Lane 4 : HeLa whole cell lysate with Human Histone H1 (tri methyl K25) peptide (ab17587) at 1 µg/ml
    Lane 5 : HeLa nuclear lysate with Human Histone H1 peptide (ab17588) at 1 µg/ml
    Lane 6 : HeLa whole cell lysate with Human Histone H1 peptide (ab17588) at 1 µg/ml
    Lane 7 : HeLa nuclear lysate with Human Histone H1 (di methyl K25) peptide (ab21998) at 1 µg/ml
    Lane 8 : HeLa whole cell lysate with Human Histone H1 (di methyl K25) peptide (ab21998) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution at 1/10000 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 17 kDa (possible cross reactivity).
  • Image courtesy of Human Protein Atlas

    ab17347 staining histone H1 tri methyl K25 in female cerebellum, showing a distinct and strong staining pattern at cells in the granular and molecular layers. Paraffin embedded human skin tissue was incubated with ab17347(1/300 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab17347 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

    .
  • ICC/IF image of ab17347 stained human HepG2 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab17347, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293, MCF7 cells.

Anti-Histone H1 (tri methyl K25) antibody (ab17347)参考文献

This product has been referenced in:
  • Agger K  et al. UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene regulation and development. Nature 449:731-4 (2007). ICC/IF ; Human . Read more (PubMed: 17713478) »

See 1 Publication for this product

Product Wall

Application ChIP
Detection step Real-time PCR
Sample Mouse Cell lysate - whole cell (primary hepatoctyes)
Specification primary hepatoctyes
Negative control Dnmt1
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control H3K27me3
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Verified customer

提交于 Oct 24 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step 3%BSA+0.1% Triton X100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
Sample Mouse Cell (primary hepatoctyes)
Specification primary hepatoctyes
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 08 2014

Thank you for your enquiry. This antibody was raised against histone H1 (H1.4; SwissProt reference P10412). This methyl-lysine is referred to as K25 and K26 in the literature. This is confusion originating from the inclusion of the methioine in the...

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Thank you for getting back to me. Yes, I would very much appreciate it if you could provide me with your blots. However, what would be more informative would be if you could determine the result by western blotting using histones methylated by G9a ...

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