Histone Demethylase KDM1/LSD1 Inhibitor Assay试剂盒(ab113456)

概述

  • 产品名称
    Histone Demethylase KDM1/LSD1 Inhibitor Assay试剂盒
    参阅全部 KDM1 / LSD1 试剂盒
  • 检测类型
    Enzyme activity
  • 检测时间
    1h 00m
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Mammal
  • 产品概述

    Histone Demethylase KDM1/LSD1 Inhibitor Assay Kit (ab113456) allows the user to measure inhibition of KDM1/LSD1. The kit is ready-to-use and provides all the essential components needed for screening KDM1/LSD1 inhibitors, which are directly interacting with he enzyme or blocking the binding to its substrate.


    KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation.

  • 经测试应用
    适用于: Functional Studiesmore details

性能

  • 存放说明
    Please refer to protocols.
  • 组件 48 tests 96 tests
    8-Well Assay Strips (with Frame) 6 units 12 units
    Fluoro Developer 1 x 12µl 1 x 24µl
    Fluoro Diluter 1 x 4ml 1 x 8ml
    Fluoro Enhancer 1 x 12µl 1 x 24µl
    LSD1 Assay Buffer 1 x 2ml 1 x 4ml
    LSD1 Assay Standard, 100 mM 1 x 10µl 1 x 20µl
    LSD1 Inhibitor, 1 mM 1 x 25µl 1 x 50µl
    LSD1 Substrate, 0.7 mM 1 x 150µl 1 x 300µl
  • 研究领域
  • 相关性
    Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2)H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor(ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development.
  • 别名
    • Amine oxidase (flavin containing) domain 2
    • AOF2
    • BHC110
    • BRAF35-HDAC complex protein BHC110
    • FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit
    • Flavin-containing amine oxidase domain-containing protein 2
    • KDM1
    • KIAA0601
    • LSD1
    • Lysine (K) specific demethylase 1
    • Lysine (K) specific demethylase 1A
    • Lysine-specific histone demethylase 1
    • Lysine-specific histone demethylase 1A
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab113456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

图片

  • Inhibition of KDM1/LSD1 by tranylcypromine (TCP) detected using ab113456. [LSD1 concentration: 500 ng/well].

实验方案

文献

ab113456 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting Abcam. For ab113459 and ab113460, both kits directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format. The main difference between the 2 are that ab113459, has a co...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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