Histone Demethylase (H3K4) Activity Quantification Assay试剂盒(ab113455)

概述

  • 产品名称
    Histone Demethylase (H3K4) Activity Quantification Assay试剂盒
    参阅全部 Histone Demethylase 试剂盒
  • 检测类型
    Enzyme activity
  • 检测时间
    3h 00m
  • 种属反应性
    与反应: Mouse, Human, Plants
    预测可用于: Mammal, Other
  • 产品概述

    Histone Demethylase (H3K4) Activity Quantification Assay Kit (ab113455) allows the user to measure activity/inhibition of H3K4 specific histone demethylases using cell/ tissue extracts. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase H3K4 specific activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.


    Methylation of histone H3 at lysine 4 (H3K4) is thought to be a global epigenetic mark in euchromatin and a mediator of activated transcription. H3K4 demethylation is regulated by KDM1/LSD1, which can remove di- and mono-methylation from H3K4, and the JARID proteins such as RBP2 which catalyzes the removal of methylation through a hydroxylation reaction.


     

  • 经测试应用
    适用于: Functional Studiesmore details

性能

应用

Our Abpromise guarantee covers the use of ab113455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

图片

  • Nuclear extracts were prepared from Hela cells using the ab113475 and H3K4 specific histone demethyase activity was measured.

实验方案

文献

ab113455 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your interest in ab113455 & ab113464.

You are correct that both these kits measure demethylase activity. The major difference between these 2 kits is the substrate captured on the well. For ab113455, the substrate is a dimet...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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