Anti-Histone Core抗体(ab7832)

概述

  • 产品名称Anti-Histone Core抗体
  • 描述
    羊多克隆抗体to Histone Core
  • 特异性The antibody binds to H1, H2B, H3 and H4. There is no evidence to suggest that the antibody can bind to H2A.
  • 经测试应用适用于: IP, WB, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Mouse, Cow, Human
  • 免疫原

    Human histone.

  • 阳性对照
    • Tonsil, colon carcinoma and any other human tissues.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Phosphate buffered saline
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
WB
ICC/IF
ELISA
  • 应用说明ELISA: 1/1000.
    ICC/IF: Use at an assay dependent dilution (PMID 18478156).
    IP: Use at an assay dependent dilution.
    WB: 1/200.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. The histone core protein is an octamer consisting of 2 subunits of each of histone 2A, 2B, 3 and 4. In conjunction with the linker histone H1 and the nonhistone chromosomal proteins, it is responsible for the binding and compacting of DNA into chromatin. The sequences of the core histones are highly conserved from species to species.
    • 细胞定位Nuclear

    Anti-Histone Core antibody 图像

    Anti-Histone Core antibody (ab7832)参考文献

    This product has been referenced in:
    • Li Y  et al. Characterization of nucleohistone and nucleoprotamine components in the mature human sperm nucleus. Asian J Androl 10:535-41 (2008). ICC/IF ; Human . Read more (PubMed: 18478156) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HL60)
    Loading amount 10 µg
    Specification HL60
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 13 2007

    The antibody (ab7832 -- Sheep polyclonal to Histone Core)binds to H1, H2B, H3 and H4; however, there is no evidence to suggest that the antibody can bind to H2A.

    This antibody has not been tested specifically in Drosophila, but it works in both animal and plant tissue, so I would expect it to work in Drosophila. Core histones are highly conserved.

    This antibody has not been tested in budding yeast, however as histones are highly conserved across species, it is quite likely that the antibody will react with yeast histones. As the antibody is raised against a mixture of histones, I think it likely...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"