Anti-Histidine decarboxylase抗体(ab37291)


  • 产品名称Anti-Histidine decarboxylase抗体
    参阅全部 Histidine decarboxylase 一抗
  • 描述
    兔多克隆抗体to Histidine decarboxylase
  • 特异性Detects both the 74 kDa full length and the 54 kDa active form of Histidine decarboxylase.
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Histidine decarboxylase. synthetic peptide around aa 22
    (Peptide available as ab55801)



Our Abpromise guarantee covers the use of ab37291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 21873469
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).Can be blocked with Histidine decarboxylase peptide (ab55801).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


Anti-Histidine decarboxylase antibody 图像

  • Predicted band size : 74 kDa
  • IHC image of ab37291 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37291, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histidine decarboxylase antibody (ab37291)参考文献

This product has been referenced in:
  • Nizamutdinova IT  et al. Involvement of histamine in endothelium-dependent relaxation of mesenteric lymphatic vessels. Microcirculation 21:640-8 (2014). Read more (PubMed: 24750494) »
  • Francis H  et al. Inhibition of histidine decarboxylase ablates the autocrine tumorigenic effects of histamine in human cholangiocarcinoma. Gut 61:753-64 (2012). ICC/IF ; Human . Read more (PubMed: 21873469) »

See all 5 Publications for this product

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Thank you for your reply. I can see that my colleagues are assisting you with ab3611 and ab15323. The protocol suggestions that I have given you are specific for ab37291 and I see that they have also offered protocol suggestions for these products. ...

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Thank you for contacting Abcam regarding ab37291. I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed the protocol information you provided and I would like to make some suggestions to improve your resu...

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