Anti-HIRIP3抗体(ab99208)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to HIRIP3
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 395-444 (RTRSSSSSSD GSPEAKGGKA GSGRRGEDHP AVMRLKRYIR ACGAHRNYKK) of Human HIRIP3 (NP_003600).

  • 阳性对照
    • ACHN cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab99208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    May play a role in chromatin function and histone metabolism via its interaction with HIRA and histones.
  • 组织特异性
    Widely expressed. Isoform 1 is predominant in skeletal muscle. Isoform 2 is predominant in liver and heart.
  • 翻译后修饰
    Phosphorylated by CK2.
  • 细胞定位
    Nucleus. Nuclear throughout the cell cycle and is excluded from condensed chromatin during mitosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HIRA-interacting protein 3 antibody
    • HIRIP3 antibody
    • HIRP3 antibody
    • HIRP3_HUMAN antibody

图片

  • Anti-HIRIP3 antibody (ab99208) at 1 µg/ml + ACHN cell lysate at 10 µg

    Predicted band size : 62 kDa

实验方案

文献

ab99208 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (different cell lines)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
40 µg
Specification
different cell lines
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 20 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF and whole brain lysate)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
40 µg
Specification
MEF and whole brain lysate
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 12 2016

Thank you for your answer.

I do completely understand your concerns. I am happy to see that the ab99208 does seem to provide finally good results. I am sorry to hear that the results with ab100856 are unsatisfactory.

Indeed, both an...

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Thank you for taking time to complete our questionnaire. I am sorry that this antibody is not providing satisfactory results.

Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab99208. I woul...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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