Synthetic peptide conjugated to KLH derived from within residues 1600 to the C-terminus of Saccharomyces cerevisiae HIR3.
(Peptide available as
This antibody gave a positive signal in S. cerevisiae Yeast Extract.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 192 kDa (predicted molecular weight: 192 kDa).
HIR3 is a member of the HIR complex that also contains HIR1, HIR2 and HPC2. The HIR complex is responsible for the repression of histone gene transcription as well having a role in nucleosome assembly.
Histone transcription regulator 3 antibody
Western blot - Anti-HIR3 antibody (ab61639)
Anti-HIR3 antibody (ab61639) at 1 µg/ml + S.cerevisiae Yeast Extract at 10 µg
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 192 kDa Observed band size: 192 kDa Additional bands at: 30 kDa, 55 kDa, 65 kDa, 75 kDa. We are unsure as to the identity of these extra bands. Exposure time: 8 minutes
has not yet been referenced specifically in any publications.
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