Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Saccharomyces cerevisiae HIR1.
(Peptide available as
This antibody gave a positive signal in Saccharomyces cerevisiae Yeast Extract.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa).
HIR1 is a member of the HIR complex that also contains HIR2, HIR3 and HPC2. This complex is responsible for the repression of histone gene transcription as well as having a role in nucleosome assembly.
Histone transcription regulator 1 antibody
Western blot - HIR1 antibody (ab46878)
Anti-HIR1 antibody (ab46878) at 1 µg/ml + S Cerevisiae Yeast Extract at 10 µg
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Performed under reducing conditions. Predicted band size: 94 kDa Observed band size: 94 kDa Additional bands at: 15 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
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