The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
功能Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.
组织特异性Present in many tissues such as brain, thymus, lymph node, lung, liver, skin and kidney (at protein level).
序列相似性Belongs to the IFT57 family.
结构域The pseudo DED region (pDED) meadiates the interaction with HIP1.
细胞定位Cytoplasm > cytoskeleton > cilium basal body. Concentrates within the inner segment of cilia.