概述

  • 产品名称Anti-HIPK4抗体
    参阅全部 HIPK4 一抗
  • 描述
    小鼠单克隆抗体to HIPK4
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Cynomolgus Monkey, Rhesus monkey
  • 免疫原

    Recombinant fragment: RGSSWEEGEH LGASAEPLAI LQRDEDGPNI DNMTMEAERP DPELFDPSSC PGEWLSEPDC TLESVRGPRA QGLPPRRSHQ HGPPRGATSF LQHVTGHH, corresponding to amino acids 519-616 of Human HIPK4 (AAH34501) with a proprietory tag.

  • 阳性对照
    • HIPK4 transfected 293T cell lysate. Recombinant tagged human HIPK4 fragment.

性能

应用

Our Abpromise guarantee covers the use of ab77651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 69 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Protein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter (By similarity). May act as a corepressor of transcription factors.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HIPK 4 antibody
    • HIPK4 antibody
    • HIPK4_HUMAN antibody
    • Homeodomain interacting protein kinase 4 antibody
    • Homeodomain-interacting protein kinase 4 antibody
    see all

Anti-HIPK4 antibody 图像

  • Anti-HIPK4 antibody (ab77651) at 1 µg/ml + Recombinant tagged human HIPK4 fragment at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 69 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-HIPK4 antibody (ab77651) at 1 µg/ml

    Lane 1 : HIPK4 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 69 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

Anti-HIPK4 antibody (ab77651)参考文献

ab77651 has not yet been referenced specifically in any publications.

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