概述

  • 产品名称Anti-HIPK2抗体
    参阅全部 HIPK2 一抗
  • 描述
    兔多克隆抗体to HIPK2
  • 特异性ab28507 recognises HIPK2. It may recognize isoform 1, 2 and 3 of HIPK2 as the immunogen sequence is present in all three isoforms. Please refer to the literature for more information on the three isforms. It does not recognize HIPK1, HIPK3, or HIPK4.
  • 经测试应用适用于: ICC/IF, ELISA, WB, IHC-P, IHC-Fr, ChIPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide derived from within residues 37 - 86 of human HIPK2.

    .

  • 阳性对照
    • HepG2 Cell Lysate, human heart tissue

性能

应用

Our Abpromise guarantee covers the use of ab28507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/62500.
WB Use a concentration of 0.06 µg/ml. Predicted molecular weight: 110 kDa.Can be blocked with Human HIPK2 peptide (ab111675). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
IHC-Fr Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 23565059

靶标

  • 功能Protein kinase acting as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Inhibits cell growth and promotes apoptosis. Involved in transcriptional activation of TP53 and TP73. Phosphorylation of TP53 may be mediated by a TP53-HIPK2-AXIN1 complex. In response to TGFB, cooperates with DAXX to activate JNK. Phosphorylates the antiapoptotic factor CTBP1 and promotes its proteasomal degradation. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between TAK1 and NLK to promote the proteasomal degradation of MYB (By similarity). Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation.
  • 组织特异性Highly expressed in heart, muscle and kidney. Weakly expressed in a ubiquitous way. Down-regulated in several thyroid and breast tumors.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated on tyrosines (By similarity). Autophosphorylated.
    Sumoylated. When conjugated it is directed to nuclear speckles. Desumoylated by SENP1 (By similarity). Sumoylation on Lys-32 is promoted by the E3 SUMO-protein ligase CBX4.
    Ubiquitinated by FBXO3, leading to rapid proteasome-dependent degradation. This degradation, but not ubiquitination, is prevented in the presence of PML.
  • 细胞定位Nucleus > PML body. Cytoplasm. Concentrated in PML/POD/ND10 nuclear bodies. Small amounts are cytoplasmic.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hHIPk 2 antibody
    • hHIPk2 antibody
    • HIPK 2 antibody
    • Hipk2 antibody
    • HIPK2_HUMAN antibody
    • Homeodomain interacting protein kinase 2 antibody
    • Homeodomain-interacting protein kinase 2 antibody
    • Nbak1 antibody
    • Nuclear body-associated kinase 1 antibody
    • PRO0593 antibody
    • Sialophorin tail-associated nuclear serine/threonine-protein kinase antibody
    • Stank antibody
    see all

Anti-HIPK2 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling HIPK2 with ab28507 at 1/100. A Cy3-conjugated donkey anti-rabbit (1/200) was used as the secondary antibody. Positive staining seen the nuclei of pinealocytes and intersticial cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - HIPK2. Right - Merge.
  • ab28507, at 4.0-8.0ug/ml, staining human HIPK2 in (Human heart, cells with positive label are Myocardial cells) by Immunohistochemistry, Paraffin embedded tissue.
  • Anti-HIPK2 antibody (ab28507) at 0.06 µg/ml + HepG2 Cell Lysate

    Secondary
    Rabbit Anti HIPK2 at 1/50000 dilution

    Predicted band size : 110 kDa
  • ICC/IF image of ab28507 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28507, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HIPK2 antibody (ab28507)参考文献

This product has been referenced in:
  • Lin J  et al. Downregulation of HIPK2 increases resistance of bladder cancer cell to cisplatin by regulating Wip1. PLoS One 9:e98418 (2014). WB ; Human . Read more (PubMed: 24846322) »
  • Shang Y  et al. Transcriptional corepressors HIPK1 and HIPK2 control angiogenesis via TGF-ß-TAK1-dependent mechanism. PLoS Biol 11:e1001527 (2013). ChIP ; Human . Read more (PubMed: 23565059) »

See all 3 Publications for this product

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I am very pleased to hear you would like to accept our offer and test ab28507 in IHC-P on mouse. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHC-P on mouse and include ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 30 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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提交于 Dec 21 2011

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse retina sections)
Specification mouse retina sections
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Nov 22 2010

Thank you for your reply. The originator of the antibody assures me that the antibody does not react with HIPK1, HIPK3, or HIPK4. I include below the exact protocol that was used to test the antibody in Western blot: To prepare total cell ly...

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Thank you for your enquiry. ab28507 recognizes HIPK2 and was tested on HepG2 cells. It may recognize isoforms 1, 2 and 3 as the immunogen sequence is present in all three isoforms. Isoform two is missing approximately 300 amino acids and would run ...

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