概述

  • 产品名称Anti-HIPK1抗体
    参阅全部 HIPK1 一抗
  • 描述
    小鼠多克隆抗体to HIPK1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Dog, Macaque Monkey
  • 免疫原

    Recombinant fragment: VRQQQSSSLP SKKNKQSAPV SSKSSLDVLP SQVYSLVGSS PLRTTSSYNS LVPVQDQHQP IIIPDTPSPP VSVITIRSDT DEEEDNKYKP SSSGLKPRSN , corresponding to amino acids 833-932 of Human HIPK1

  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab43641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 131 kDa).

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能May play a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. May be involved in malignant squamous cell tumor formation.
  • 组织特异性Ubiquitously expressed with highest levels in skeletal muscle and heart. Overexpressed in breast cancer cell lines.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated. Phosphorylated and activated by JNK1.
  • 细胞定位Nucleus. Cytoplasm. Predominantly nuclear.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Hipk 1 antibody
    • Hipk1 antibody
    • HIPK1_HUMAN antibody
    • Homeodomain interacting protein kinase 1 antibody
    • Homeodomain interacting protein kinase 1 like protein antibody
    • Homeodomain-interacting protein kinase 1 antibody
    • Myak antibody
    • Nbak 2 antibody
    • Nbak2 antibody
    • Nuclear body associated kinase 2 antibody
    • Nuclear body associated kinase 2b antibody
    see all

Anti-HIPK1 antibody 图像

  • All lanes : Anti-HIPK1 antibody (ab43641) at 1/1000 dilution

    Lane 1 : ~20ug of a total protein extract from E coli containing 50ng of a tagged fusion protein of an irrelevant antigen
    Lane 2 : ~20ug of a total protein extract from E coli with 50ng of the antigen (tagged fusion protein).

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 5000

    Predicted band size : 131 kDa

Anti-HIPK1 antibody (ab43641)参考文献

ab43641 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab43641.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"