RabMAb

Anti-HIP2抗体[EP1144Y] (ab68162)

概述

  • 产品名称Anti-HIP2抗体[EP1144Y]
    参阅全部 HIP2 一抗
  • 描述
    兔单克隆抗体[EP1144Y] to HIP2
  • 经测试应用适用于: WB, IP, ICCmore details
    不适用于: Flow Cyt or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues on the N-terminus of human HIP2.

  • 阳性对照
    • Daudi, Jurkat, HepG2 and human brain cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab68162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
IP 1/20.
ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IHC-P.
  • 靶标

    • 功能Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
    • 组织特异性Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem.
    • 通路Protein modification; protein ubiquitination.
    • 序列相似性Belongs to the ubiquitin-conjugating enzyme family.
      Contains 1 UBA domain.
    • 翻译后修饰Sumoylation at Lys-14 impairs catalytic activity.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • E2 25K antibody
      • E2(25K) antibody
      • HIP-2 antibody
      • Huntingtin interacting protein 2 antibody
      • Huntingtin-interacting protein 2 antibody
      • HYPG antibody
      • LIG antibody
      • UBC1 antibody
      • UBE2K antibody
      • UBE2K_HUMAN antibody
      • Ubiquitin carrier protein antibody
      • Ubiquitin conjugating enzyme E2 25 kDa antibody
      • ubiquitin conjugating enzyme E2K antibody
      • ubiquitin conjugating enzyme E2K (UBC1 homolog, yeast) antibody
      • Ubiquitin protein ligase antibody
      • Ubiquitin-conjugating enzyme E2 K antibody
      • Ubiquitin-conjugating enzyme E2(25K) antibody
      • Ubiquitin-conjugating enzyme E2-25 kDa antibody
      • Ubiquitin-conjugating enzyme E2-25K antibody
      • Ubiquitin-protein ligase antibody
      see all

    Anti-HIP2 antibody [EP1144Y] 图像

    • All lanes : Anti-HIP2 antibody [EP1144Y] (ab68162) at 1/500 dilution

      Lane 1 : Daudi cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : human brain cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 22 kDa
      Observed band size : 25 kDa (why is the actual band size different from the predicted?)

    Anti-HIP2 antibody [EP1144Y] (ab68162)参考文献

    ab68162 has not yet been referenced specifically in any publications.

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