Anti-HIP2/LIG抗体[757C3a] (ab50837)
Key features and details
- Mouse monoclonal [757C3a] to HIP2/LIG
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-HIP2/LIG抗体[757C3a]
参阅全部 HIP2/LIG 一抗 -
描述
小鼠单克隆抗体[757C3a] to HIP2/LIG -
宿主
Mouse -
特异性
This antibody reacts with HIP2/LIG.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human HIP2/LIG.
Database link: P61086 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Protein G purified from cultured supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum, filtered through 0.22µm membrane. -
克隆
单克隆 -
克隆编号
757C3a -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50837于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 32 kDa (predicted molecular weight: 22 kDa).
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 32 kDa (predicted molecular weight: 22 kDa). |
靶标
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功能
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1. -
组织特异性
Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the ubiquitin-conjugating enzyme family.
Contains 1 UBA domain. -
翻译后修饰
Sumoylation at Lys-14 impairs catalytic activity. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 3093 Human
- Omim: 602846 Human
- SwissProt: P61086 Human
- Unigene: 50308 Human
- Unigene: 725263 Human
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别名
- E2 25K antibody
- E2(25K) antibody
- HIP-2 antibody
see all
图片
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Anti-HIP2/LIG antibody [757C3a] (ab50837) + Recombinant HIP2/LIG immunizing protein.
Predicted band size: 22 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (0)
ab50837 尚未被引用在任何文献中。