Anti-HINT3抗体(ab121960)

概述

  • 产品名称Anti-HINT3抗体
  • 描述
    兔多克隆抗体to HINT3
  • 经测试应用适用于: IP, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: MAEEQVNRSA GLAPDCEASA TAETTVSSVG TCEAAAKSPE PKDYDSTCVF CRIAGRQDPG TELLHCENED LICFKDIKPA ATHHYLVVP, corresponding to amino acids 1-89 of Human HINT3 (UniProt Q9NQE9).

  • 阳性对照
    • WB: RT4, U-251 MG, Human liver and Human tonsil lysates IHC-P: Human colon tissue ICC/IF: U-2 OS cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval via the pressure cooker method.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

靶标

Anti-HINT3 antibody 图像

  • All lanes : Anti-HINT3 antibody (ab121960) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique
  • ab121960, at a 1/100 dilution, staining HINT3 in paraffin emebedded Human colon tissue by Immunohistochemistry.
  • ab121960, at 4 µg/ml, staining HINT3 in Human cell line U-2 OS by Immunofluorescence.

Anti-HINT3 antibody (ab121960)参考文献

ab121960 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Sample Escherichia coli Cell lysate - whole cell (E.coli cell lysate)
Total protein in input 2000 µg
Specification E.coli cell lysate
Immuno-precipitation step Other - Dynabeads
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提交于 Sep 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"