RabMAb

Anti-HINT1抗体[EPR5108] (ab124912)

概述

  • 产品名称Anti-HINT1抗体[EPR5108]
    参阅全部 HINT1 一抗
  • 描述
    兔单克隆抗体[EPR5108] to HINT1
  • 经测试应用适用于: WB, IP, IHC-P, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human HINT1.

  • 阳性对照
    • 293T, MCF7, HepG2 and Jurkat cell lysates; Human breast carcinoma and Human colonic adenocarcinoma tissues.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the HINT family.
    Contains 1 HIT domain.
  • 结构域The histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide.
  • 细胞定位Cytoplasm. Nucleus. Interaction with CDK7 leads to a more nuclear localization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adenosine 5' monophosphoramidase antibody
    • Adenosine 5''-monophosphoramidase antibody
    • HINT 1 antibody
    • HINT1 antibody
    • HINT1_HUMAN antibody
    • Histidine triad nucleotide binding protein 1 antibody
    • Histidine triad nucleotide-binding protein 1 antibody
    • PKCI 1 antibody
    • PKCI-1 antibody
    • PKCI1 antibody
    • PRKCNH1 antibody
    • Protein kinase C inhibitor 1 antibody
    • Protein kinase C interacting protein 1 antibody
    • Protein kinase C-interacting protein 1 antibody
    see all

Anti-HINT1 antibody [EPR5108] 图像

  • All lanes : Anti-HINT1 antibody [EPR5108] (ab124912) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 14 kDa
  • ab124912, at 1/250 dilution, staining HINT1 in paraffin embedded breast carcinoma tissue by immunohistochemistry.
  • ab124912, at 1/250 dilution, staining HINT1 in paraffin embedded colonic adenocarcinoma tissue by immunohistochemistry.

Anti-HINT1 antibody [EPR5108] (ab124912)参考文献

ab124912 has not yet been referenced specifically in any publications.

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