antigen sequence: MATLGFVTPE APFESSKPPI FEGLSPTVYS NPEGFKEKFL RKTRENP, corresponding to N terminal amino acids 1-47 of Human HIGD2B
- Human heart muscle tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
Multipass membrane protein.
Contains 1 HIG1 domain.
- HIG1 domain family member 2B pseudogene antibody
- HIGD2BP antibody
Immunocytochemistry/ Immunofluorescence - Anti-HIGD2B antibody (ab121586)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121586 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIGD2B antibody (ab121586)
ab121586, at a 1/200 dilution, staining HIGD2BP in myocytes of paraffin-embedded Human heart muscle tissue by immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"