Anti-HIGD1A抗体(ab117456)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to HIGD1A
  • 特异性
    ab117456 is predicted to not cross-react with HIG2.
  • 经测试应用
    适用于: ICC/IF, ICC, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to 19 amino acids near the N terminal of Human HIGD1A (NP_001093138).

  • 阳性对照
    • 293 cell lysate.
  • 常规说明
    Avoid repeated freeze thaw cycles and exposure to prolonged high temperatures.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab117456 was purified by affinity chromatography via a peptide column.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab117456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
ICC Use a concentration of 2.5 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 12 kDa.

靶标

图片

  • Immunofluorescence of HIGD1A in 293 cells tissue with ab117456 at 10 ug/mL.
  • Lane 1 : Anti-HIGD1A antibody (ab117456) at 0.5 µg/ml
    Lane 2 : Anti-HIGD1A antibody (ab117456) at 1 µg/ml

    Lane 1 : 293 cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 12 kDa
  • Immunocytochemical analysis of 293 cells, staining HIGD1A with ab117456 at 2.5 µg/ml.

文献

ab117456 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (C2C12, MEF)
Loading amount
25 µg
Specification
C2C12, MEF
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 23 2012

Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2, 293T)
Loading amount
0 µg
Specification
HepG2, 293T
Gel Running Conditions
Reduced Denaturing (15% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 23 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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