概述

  • 产品名称Anti-HIF3 alpha抗体
    参阅全部 HIF3 alpha 一抗
  • 描述
    兔多克隆抗体to HIF3 alpha
  • 特异性We have observed both nuclear and cytoplasmic localisation in IF with this antibody and we believe this could be due to dysregulated expression of HIF3 alpha in our cell lines.
  • 经测试应用适用于: WB, ICC/IF, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human HIF3 alpha.

    (Peptide available as ab20077.)

  • 阳性对照
    • This antibody gives a positive signal in human heart tissue lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab10134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 72 kDa).Can be blocked with Human HIF3 alpha peptide (ab20077).
ICC/IF Use a concentration of 1 - 5 µg/ml.
IP Use at an assay dependent concentration.
IHC-P 1/100.

靶标

  • 功能Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea.
  • 组织特异性Expressed in kidney. Expressed abundantly in lung epithelial cells. Expression is regulated in an oxygen-dependent manner.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • 翻译后修饰In normoxia, hydroxylated on Pro-492 in the oxygen-dependent degradation domain (ODD) by PHD. The hydroxylated proline promotes interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation.
  • 细胞定位Nucleus. Cytoplasm. In the nuclei of all periportal and perivenous hepatocytes. In the distal perivenous zone, detected in the cytoplasm of the hepatocytes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Basic-helix-loop-helix-PAS protein MOP7 antibody
    • bHLHe17 antibody
    • Class E basic helix-loop-helix protein 17 antibody
    • HIF 3A antibody
    • HIF 3A4 antibody
    • HIF-3-alpha antibody
    • HIF3 alpha antibody
    • HIF3-alpha antibody
    • HIF3-alpha-1 antibody
    • HIF3A antibody
    • HIF3A_HUMAN antibody
    • Hypoxia Inducible Factor 3 alpha antibody
    • Hypoxia inducible factor 3 alpha subunit antibody
    • Hypoxia inducible factor three alpha antibody
    • Hypoxia-inducible factor 3-alpha antibody
    • Inhibitory PAS domain protein antibody
    • IPAS antibody
    • Member of PAS protein 7 antibody
    • MOP7 antibody
    • PAS domain-containing protein 7 antibody
    • PASD7 antibody
    see all

Anti-HIF3 alpha antibody 图像

  • ICC/IF image of ab10134 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab10134, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • ICC/IF image of ab10134 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10134, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Anti-HIF3 alpha antibody (ab10134) at 2 µg/ml + Human heart tissue lysate - total protein (ab29431) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 72 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes

Anti-HIF3 alpha antibody (ab10134)参考文献

This product has been referenced in:
  • Gits CM  et al. MicroRNA response to hypoxic stress in soft tissue sarcoma cells: microRNA mediated regulation of HIF3a. BMC Cancer 14:429 (2014). WB ; Human . Read more (PubMed: 24927770) »
  • Elbarghati L  et al. Effects of hypoxia on transcription factor expression in human monocytes and macrophages. Immunobiology 213:899-908 (2008). WB ; Human . Read more (PubMed: 18926304) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (small bowel adenoma)
Specification small bowel adenoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jul 31 2007

Thank you for your enquiry. Do you know the name of the person who does the purchasing from your lab? I will only be able to give you a replacement for the antibody. We can only replace or refund the product that was defective, typically only with...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (in vitro translated HIF-3a1)
Specification in vitro translated HIF-3a1
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Username

Abcam user community

Verified customer

提交于 Feb 22 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - other (IVT HIF-3a1)
Specification IVT HIF-3a1
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

提交于 Feb 07 2006

Thank you for your enquiry. I blasted the immunogen sequence and found that the sequence is identical in isoforms a, b, c, and d. I was unable to find the other two splice variants. If you could send me the sequences of the other two isoforms, I c...

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Thank you very much for providing the details of your protocol. I would like to try RIPA buffer which contains the ionic detergent sodium deoxycholate as an active constituent and is particularly used for nuclear membrane disruption for nuclear ext...

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Thank you for your email and I'm sorry to hear that you are experiencing difficulty with ab10134. Could we get a detailed protocol from you, please? We have some general questions, the answers to which will enable us to investigate this matter as quick...

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