概述

  • 产品名称Anti-HIF1AN抗体[FIH162C]
    参阅全部 HIF1AN 一抗
  • 描述
    小鼠单克隆抗体[FIH162C] to HIF1AN
  • 经测试应用适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human FIH expressed in Escherichia coli BL21(DE3) cells.

  • 阳性对照
    • MCF7 cells.

性能

应用

Our Abpromise guarantee covers the use of ab103401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.
  • 序列相似性Contains 1 JmjC domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp762F1811 antibody
    • Factor inhibiting HIF-1 antibody
    • Factor inhibiting HIF1 antibody
    • FIH 1 antibody
    • FIH-1 antibody
    • FIH1 antibody
    • FLJ20615 antibody
    • FLJ22027 antibody
    • HIF1AN antibody
    • HIF1N_HUMAN antibody
    • Hypoxia inducible factor 1 alpha inhibitor antibody
    • Hypoxia inducible factor 1 alpha subunit inhibitor antibody
    • Hypoxia inducible factor asparagine hydroxylase antibody
    • Hypoxia-inducible factor 1-alpha inhibitor antibody
    • Hypoxia-inducible factor asparagine hydroxylase antibody
    • Peptide aspartate beta dioxygenase antibody
    see all

Anti-HIF1AN antibody [FIH162C] 图像

  • Overlay histogram showing Jurkat cells stained with ab103401 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab103401, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-HIF1AN antibody [FIH162C] (ab103401)参考文献

This product has been referenced in:
  • Chan MC  et al. Tuning the Transcriptional Response to Hypoxia by Inhibiting Hypoxia-inducible Factor (HIF) Prolyl and Asparaginyl Hydroxylases. J Biol Chem 291:20661-73 (2016). Human . Read more (PubMed: 27502280) »
  • Soilleux EJ  et al. Use of novel monoclonal antibodies to determine the expression and distribution of the hypoxia regulatory factors PHD-1, PHD-2, PHD-3 and FIH in normal and neoplastic human tissues. Histopathology 47:602-10 (2005). Read more (PubMed: 16324198) »

See all 3 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab103401.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"