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概述

  • 产品名称
    Anti-HIF1AN抗体[FIH162C]
    参阅全部 HIF1AN 一抗
  • 描述
    小鼠单克隆抗体[FIH162C] to HIF1AN
  • 经测试应用
    适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human FIH expressed in Escherichia coli BL21(DE3) cells.

  • 阳性对照
    • MCF7 cells.

性能

应用

Our Abpromise guarantee covers the use of ab103401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.
  • 序列相似性
    Contains 1 JmjC domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
    • 别名
      • DKFZp762F1811 antibody
      • Factor inhibiting HIF-1 antibody
      • Factor inhibiting HIF1 antibody
      • FIH 1 antibody
      • FIH-1 antibody
      • FIH1 antibody
      • FLJ20615 antibody
      • FLJ22027 antibody
      • HIF1AN antibody
      • HIF1N_HUMAN antibody
      • Hypoxia inducible factor 1 alpha inhibitor antibody
      • Hypoxia inducible factor 1 alpha subunit inhibitor antibody
      • Hypoxia inducible factor asparagine hydroxylase antibody
      • Hypoxia-inducible factor 1-alpha inhibitor antibody
      • Hypoxia-inducible factor asparagine hydroxylase antibody
      • Peptide aspartate beta dioxygenase antibody
      see all

    Anti-HIF1AN antibody [FIH162C] 图像

    • Flow Cytometry-Anti-HIF1AN antibody [FIH162C](ab103401)
      Flow Cytometry-Anti-HIF1AN antibody [FIH162C](ab103401)
      Overlay histogram showing Jurkat cells stained with ab103401 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab103401, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Anti-HIF1AN antibody [FIH162C] (ab103401)参考文献

    This product has been referenced in:
    • Chan MC  et al. Tuning the Transcriptional Response to Hypoxia by Inhibiting Hypoxia-inducible Factor (HIF) Prolyl and Asparaginyl Hydroxylases. J Biol Chem 291:20661-73 (2016). Human . Read more (PubMed: 27502280) »
    • Soilleux EJ  et al. Use of novel monoclonal antibodies to determine the expression and distribution of the hypoxia regulatory factors PHD-1, PHD-2, PHD-3 and FIH in normal and neoplastic human tissues. Histopathology 47:602-10 (2005). Read more (PubMed: 16324198) »

    See all 3 Publications for this product

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