概述

  • 产品名称Anti-HIF1AN抗体[EPR3658]
    参阅全部 HIF1AN 一抗
  • 描述
    兔单克隆抗体[EPR3658] to HIF1AN
  • 经测试应用适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human HIF1AN.

  • 阳性对照
    • Jurkat, 293T and A375 cell lysates; Human breast carcinoma and brain tissue; HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

性能

应用

Our Abpromise guarantee covers the use of ab92498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
Flow Cyt
ICC/IF
  • 应用说明Flow Cyt: 1/100.
    ICC/IF: 1/100 - 1/250.
    IHC-P: 1/250 - 1/500.
    WB: 1/5000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

    Is unsuitable for IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.
    • 序列相似性Contains 1 JmjC domain.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp762F1811 antibody
      • Factor inhibiting HIF-1 antibody
      • Factor inhibiting HIF1 antibody
      • FIH 1 antibody
      • FIH-1 antibody
      • FIH1 antibody
      • FLJ20615 antibody
      • FLJ22027 antibody
      • HIF1AN antibody
      • HIF1N_HUMAN antibody
      • Hypoxia inducible factor 1 alpha inhibitor antibody
      • Hypoxia inducible factor 1 alpha subunit inhibitor antibody
      • Hypoxia inducible factor asparagine hydroxylase antibody
      • Hypoxia-inducible factor 1-alpha inhibitor antibody
      • Hypoxia-inducible factor asparagine hydroxylase antibody
      • Peptide aspartate beta dioxygenase antibody
      see all

    Anti-HIF1AN antibody [EPR3658] 图像

    • All lanes : Anti-HIF1AN antibody [EPR3658] (ab92498) at 1/5000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : A375 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 40 kDa
      Observed band size : 40 kDa
    • ab92498 at 1/250 dilution staining HIF1AN in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
    • ab92498 at 1/250 dilution staining HIF1AN in Human brain by Immunohistochemistry, Paraffin-embedded tissue.
    • ab92498 at 1/100 dilution staining HIF1AN in HeLa cells by Immunofluorescence.

    Anti-HIF1AN antibody [EPR3658] (ab92498)参考文献

    This product has been referenced in:
    • Chen T  et al. Factor inhibiting HIF1a (FIH-1) functions as a tumor suppressor in human colorectal cancer by repressing HIF1a pathway. Cancer Biol Ther 16:244-52 (2015). WB ; Human . Read more (PubMed: 25602156) »
    • Wang E  et al. The role of factor inhibiting HIF (FIH-1) in inhibiting HIF-1 transcriptional activity in glioblastoma multiforme. PLoS One 9:e86102 (2014). IHC ; Human . Read more (PubMed: 24465898) »

    See all 2 Publications for this product

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