Anti-HIF1AN/FIH-1抗体(ab233141)
Key features and details
- Rabbit polyclonal to HIF1AN/FIH-1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
概述
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产品名称
Anti-HIF1AN/FIH-1抗体
参阅全部 HIF1AN/FIH-1 一抗 -
描述
兔多克隆抗体to HIF1AN/FIH-1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human, Pig
预测可用于: Mouse -
免疫原
Recombinant full length protein (His-T7-tag) corresponding to Human HIF1AN/FIH-1 aa 1 to the C-terminus. Expressed in E.coli. N-terminal tags.
Database link: Q9NWT6 -
阳性对照
- IHC-P: Human prostate, stomach cancer, stomach and skin cancer tissues. ICC/IF: HeLa cells. WB: HeLa and HEK-293T cell lysates; Pig kidney and brain lysates; Recombinant human HIF1AN/FIH-1 protein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
ab233141 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab233141于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 40 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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ICC/IF |
Use a concentration of 5 - 20 µg/ml.
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说明 |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 40 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
ICC/IF
Use a concentration of 5 - 20 µg/ml. |
靶标
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功能
Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. -
序列相似性
Contains 1 JmjC domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Clone ID: BC007719 Human
- Entrez Gene: 55662 Human
- Entrez Gene: 319594 Mouse
- Entrez Gene: 100155854 Pig
- Omim: 606615 Human
- SwissProt: Q9NWT6 Human
- SwissProt: Q8BLR9 Mouse
- Unigene: 500788 Human
see all -
别名
- DKFZp762F1811 antibody
- Factor inhibiting HIF-1 antibody
- Factor inhibiting HIF1 antibody
see all
图片
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All lanes : Anti-HIF1AN/FIH-1 antibody (ab233141) at 2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Pig kidney lysate
Lane 4 : Pig brain lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 40 kDa -
Paraffin-embedded human prostate tissue stained for HIF1AN/FIH-1 using ab233141 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Paraffin-embedded human stomach cancer tissue stained for HIF1AN/FIH-1 using ab233141 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Paraffin-embedded human stomach tissue stained for HIF1AN/FIH-1 using ab233141 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Paraffin-embedded human skin cancer tissue stained for HIF1AN/FIH-1 using ab233141 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HIF1AN/FIH-1 (green) using ab233141 at 20 µg/ml in ICC/IF, followed by FITC staining.
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Anti-HIF1AN/FIH-1 antibody (ab233141) at 2 µg/ml + Recombinant human HIF1AN/FIH-1 protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 40 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab233141 尚未被引用在任何文献中。