Anti-HIF1 beta抗体- ChIP Grade (ab2)

概述

  • 产品名称Anti-HIF1 beta抗体- ChIP Grade
    参阅全部 HIF1 beta 一抗
  • 描述
    兔多克隆抗体to HIF1 beta - ChIP Grade
  • 特异性This antibody is specific for HIF-1 beta/ARNT. It is not known if ab2 crossreacts with ARNT2 (which is related but a different gene product).
  • 经测试应用适用于: ChIP/Chip, IP, WB, ChIP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Cow, Human, Ferret
  • 免疫原

    Fusion protein, containing amino acids 496-789 of Human HIF-1 beta.

  • 阳性对照
    • MCF7 human nuclear extracts

应用

Our Abpromise guarantee covers the use of ab2 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP/Chip Use at an assay dependent concentration. PubMed: 20395365
IP Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 92 kDa. Background non-specific bands are minimal on MCF7 human nuclear extracts. Background may increase with whole cell extract preparations.
ChIP 1/10 - 1/500.

A customer used in a ChIP assay after purifying this antibody and applyed 0.2 µg/µg chromatin. Please see Genpathway's abreview.

ICC/IF 1/500. PubMed: 25422886

靶标

  • 功能Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • arnT antibody
    • ARNT protein antibody
    • ARNT_HUMAN antibody
    • Aryl hydrocarbon receptor nuclear translocator antibody
    • bHLHe2 antibody
    • Class E basic helix-loop-helix protein 2 antibody
    • Dioxin receptor antibody
    • Dioxin receptor nuclear translocator antibody
    • Drnt antibody
    • HIF 1 beta antibody
    • HIF 1beta antibody
    • HIF-1-beta antibody
    • HIF1-beta antibody
    • HIF1B antibody
    • HIF1beta antibody
    • Hypoxia Inducible Factor 1 antibody
    • Hypoxia inducible factor 1 beta antibody
    • Hypoxia-inducible factor 1-beta antibody
    • nuclear translocator antibody
    • Tango antibody
    see all

Anti-HIF1 beta antibody - ChIP Grade 图像

  • Anti-HIF1 beta antibody - ChIP Grade (ab2) + Hypoxic HeLa lysate at 0.5 mg/ml

    Performed under reducing conditions.

    Predicted band size : 92 kDa
  • X-ChIP with ab2 showing enrichment of HIF1 beta at a region upstream of transcription start site of the VEGFA gene. Chromatin was extracted from a glioma cell line and the proteins were cross-linked to DNA with formaldehyde for 15 minutes. The immunoprecipitation step involved incubation of the cross-linked chromatin with ab2 for 16 hours at 4°C. Please see abreview by Genpathway for additional details.

    See Abreview

Anti-HIF1 beta antibody - ChIP Grade (ab2)参考文献

This product has been referenced in:
  • Øvrevik J  et al. AhR and Arnt differentially regulate NF-?B signaling and chemokine responses in human bronchial epithelial cells. Cell Commun Signal 12:48 (2014). Human . Read more (PubMed: 25201625) »
  • Vavilala DT  et al. Evaluation of anti-HIF and anti-angiogenic properties of honokiol for the treatment of ocular neovascular diseases. PLoS One 9:e113717 (2014). IF ; Human . Read more (PubMed: 25422886) »

See all 3 Publications for this product

Product Wall


For ab2769, ChIP was added to the datasheet based on customer feedback received by the originating lab:

1. ChIP assay: The cross-linked, sheared chromatin solution was used for immunoprecipitation with 5 μl of anti-AHR antibody ab2...

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I am very pleased to hear you would like to test ab2 in flow cytometry (FC). This code will give you: 1 free primary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forFC and include this code in the “Additiona...

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Vielen Dank für Ihren Anruf.

Es tut mir leid, dass Sie solche Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen, habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 1137139 und sollt...

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Thank you for the detailed information about your experiment.

High background - as you see in your IP blot - can often come from the secondary antibody binding non-specifically to the IP antibody's heavy and light chainas well as other prote...

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - nuclear (Glioma cell line)
Specification Glioma cell line
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step Real-time PCR
Positive control Region upstream of the transcription start site of the VEGFA gene
Negative control Region on chromosome 4 (Untr4) that is far from any known gene annotation and not expected to be bound by HIF1 beta
Region on chromosome 12 (Untr12) that is far from any known gene annotation and not expected to be bound by HIF1 beta
Username

Mr. Genpathway Inc

Verified customer

提交于 Apr 27 2009

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Human Cell (Eye)
Specification Eye
Permeabilization Yes - Triton X-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 31 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"