重组
RabMAb

Anti-HIF Prolyl Hydroxylases抗体[EPR3664(2)] (ab109734)

概述

  • 产品名称
    Anti-HIF Prolyl Hydroxylases抗体[EPR3664(2)]
    参阅全部 HIF Prolyl Hydroxylases 一抗
  • 描述
    兔单克隆抗体[EPR3664(2)] to HIF Prolyl Hydroxylases
  • 经测试应用
    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human HIF Prolyl Hydroxylases.

  • 阳性对照
    • 293T, U87-MG, HeLa, HepG2, and SH-SY5Y cell lysates, Human brain tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab109734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 57 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 功能
      Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
    • 组织特异性
      Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts.
    • 序列相似性
      Contains 2 EF-hand domains.
      Contains 1 Fe2OG dioxygenase domain.
    • 翻译后修饰
      Glycosylated.
    • 细胞定位
      Endoplasmic reticulum membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EGLN4 antibody
      • FLJ20262 antibody
      • HIF prolyl hydroxylase PH4 antibody
      • HIF-PH4 antibody
      • HIF-prolyl hydroxylase 4 antibody
      • HIFPH4 antibody
      • HPH-4 antibody
      • Hypoxia inducible factor prolyl 4 hydroxylase antibody
      • Hypoxia inducible factor prolyl hydroxylase antibody
      • Hypoxia-inducible factor prolyl hydroxylase 4 antibody
      • P4H TM antibody
      • P4H with transmembrane domain antibody
      • P4H-TM antibody
      • P4htm antibody
      • P4HTM_HUMAN antibody
      • PH 4 antibody
      • PH4 antibody
      • PHD4 antibody
      • Proline 4 hydroxylase antibody
      • Prolyl 4 hydroxylase transmembrane (endoplasmic reticulum) antibody
      • Prolyl hydroxlase domain containing 4 antibody
      • Transmembrane prolyl 4-hydroxylase antibody
      see all

    图片

    • All lanes : Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)] (ab109734) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : U87-MG cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 57 kDa
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109734 at a dilution of 1/100.

    文献

    ab109734 has not yet been referenced specifically in any publications.

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    There are currently no Customer reviews or Questions for ab109734.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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