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Synthetic peptide corresponding to Human HIF-1 alpha.
HIF-1 alpha can be a difficult target to work with so we have compiled a summary of all the important information you need to know including useful tips. This can be found in the protocols tab or alternatively click here to download it.
Our Abpromise guarantee covers the use of ab72775 in the following tested applications.
|WB||1/200 - 1/1000. Predicted molecular weight: 93 kDa.|
ICC/IF image of ab72775 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72775, 1/50 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Image from Wiley M et al., J Biol Chem. 2010 Aug 27;285(35):26852-60. Epub 2010 Jun 25. Fig 2.; doi: 10.1074/jbc.M110.147041; August 27, 2010 The Journal of Biological Chemistry, 285, 26852-26860.Representative western blot detecting HIF1 alpha (Hydroxy P402) with ab72775; a different antibody was used to detect total HIF1 alpha and an antibody against actin was used as a loading control.