概述

  • 产品名称Anti-HIC5抗体
    参阅全部 HIC5 一抗
  • 描述
    兔多克隆抗体to HIC5
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (PRSGAPKERP AEPLTPPPSY GHQPQTGSGE SSGASGDKDH LYSTVCKPRS) of human HIC5 (NP_057011).

  • 阳性对照
    • Transfected 293T cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab83273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane hence regulating their activity. In the nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid, androgen, mineralocorticoid and progesterone receptor transcriptional activity. May play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA-binding activity.
  • 组织特异性Expressed in platelets, smooth muscle and prostate stromal cells (at protein level).
  • 序列相似性Belongs to the paxillin family.
    Contains 4 LIM zinc-binding domains.
  • 结构域The LIM zinc-binding domains mediate glucocorticoid receptor coactivation and interaction with AR, CRIP2, ILK, LIMS1, NR3C1, PPARG, TCF3, TCF7L2, SLC6A3 and SMAD3. The LIM zinc-binding 2 and LIM zinc-binding 3 domains mediate targeting to focal adhesions and actin stress fibers. The LIM zinc-binding 3 and LIM zinc-binding 4 domains mediate interaction with TRAF4 and MAPK15. The LIM zinc-binding 4 domain mediates interaction with HSPB1, homooligomerization and targeting to the nuclear matrix. The LIM zinc-binding 3 domain mediates interaction with PTPN12.
    The LD (leucine and aspartate-rich) motif 3 mediates interaction with GIT1 and functions as a nuclear export signal.
  • 翻译后修饰Phosphorylated by gonadotropin-releasing hormone-activated SRC.
  • 细胞定位Cell junction > focal adhesion. Nucleus matrix. Cytoplasm > cytoskeleton. Associated with the actin cytoskeleton; colocalizes with stress fibers.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Androgen receptor coactivator 55 kDa protein antibody
    • Androgen receptor coactivator ARA55 antibody
    • Androgen receptor-associated protein of 55 kDa antibody
    • ARA55 antibody
    • Hic-5 antibody
    • Hydrogen peroxide-inducible clone 5 protein antibody
    • Tgfb1i1 antibody
    • TGFI1_HUMAN antibody
    • Transforming growth factor beta 1 induced transcript 1 antibody
    • Transforming growth factor beta-1-induced transcript 1 protein antibody
    • TSC 5 antibody
    see all

Anti-HIC5 antibody 图像

  • Anti-HIC5 antibody (ab83273) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling HIC5 with ab83273 at 5.0µg/ml.

Anti-HIC5 antibody (ab83273)参考文献

ab83273 has not yet been referenced specifically in any publications.

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