概述

  • 产品名称Anti-HIC5抗体
    参阅全部 HIC5 一抗
  • 描述
    兔多克隆抗体to HIC5
  • 经测试应用适用于: ICC/IF, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Cow, Dog
  • 免疫原

    Synthetic peptide (Human). More details of the immunogen are not available.

应用

Our Abpromise guarantee covers the use of ab42476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 48 kDa.

Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ELISA 1/12500.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane hence regulating their activity. In the nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid, androgen, mineralocorticoid and progesterone receptor transcriptional activity. May play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA-binding activity.
  • 组织特异性Expressed in platelets, smooth muscle and prostate stromal cells (at protein level).
  • 序列相似性Belongs to the paxillin family.
    Contains 4 LIM zinc-binding domains.
  • 结构域The LIM zinc-binding domains mediate glucocorticoid receptor coactivation and interaction with AR, CRIP2, ILK, LIMS1, NR3C1, PPARG, TCF3, TCF7L2, SLC6A3 and SMAD3. The LIM zinc-binding 2 and LIM zinc-binding 3 domains mediate targeting to focal adhesions and actin stress fibers. The LIM zinc-binding 3 and LIM zinc-binding 4 domains mediate interaction with TRAF4 and MAPK15. The LIM zinc-binding 4 domain mediates interaction with HSPB1, homooligomerization and targeting to the nuclear matrix. The LIM zinc-binding 3 domain mediates interaction with PTPN12.
    The LD (leucine and aspartate-rich) motif 3 mediates interaction with GIT1 and functions as a nuclear export signal.
  • 翻译后修饰Phosphorylated by gonadotropin-releasing hormone-activated SRC.
  • 细胞定位Cell junction > focal adhesion. Nucleus matrix. Cytoplasm > cytoskeleton. Associated with the actin cytoskeleton; colocalizes with stress fibers.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Androgen receptor coactivator 55 kDa protein antibody
    • Androgen receptor coactivator ARA55 antibody
    • Androgen receptor-associated protein of 55 kDa antibody
    • ARA55 antibody
    • Hic-5 antibody
    • Hydrogen peroxide-inducible clone 5 protein antibody
    • Tgfb1i1 antibody
    • TGFI1_HUMAN antibody
    • Transforming growth factor beta 1 induced transcript 1 antibody
    • Transforming growth factor beta-1-induced transcript 1 protein antibody
    • TSC 5 antibody
    see all

Anti-HIC5 antibody 图像

  • Anti-HIC5 antibody (ab42476) at 1 µg/ml + Jurkat cell lysate

    Predicted band size : 48 kDa
  • Ab42476 at 4.0µg/ml staining Alveolar cells of human lung. Paraffin embedded tissue. Magnification 400X
  • ICC/IF image of ab42476 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42476, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HIC5 antibody (ab42476)参考文献

This product has been referenced in:
  • Hornigold N  et al. Inhibition of collagen I accumulation reduces glomerulosclerosis by a Hic-5-dependent mechanism in experimental diabetic nephropathy. Lab Invest 93:553-65 (2013). Rat . Read more (PubMed: 23508044) »
  • Hornigold N  et al. Upregulation of Hic-5 in glomerulosclerosis and its regulation of mesangial cell apoptosis. Kidney Int 77:329-38 (2010). IHC-P ; Rat . Read more (PubMed: 20010548) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Sample Mouse Tissue sections (uterus at 7.5 day of pregnancy)
Specification uterus at 7.5 day of pregnancy
Permeabilization No
Fixative Methanol
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提交于 Nov 07 2014

Application Immunoprecipitation
Immuno-precipitation step Other - Dynabeads
Sample Human Cell lysate - whole cell (endothelial)
Specification endothelial
Total protein in input 3e+006 cells
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提交于 Feb 13 2014

Application ChIP
Sample Mouse Cell lysate - nuclear (myeloid progenitors)
Specification myeloid progenitors
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 12 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step Real-time PCR
Positive control my gene of interest
Negative control Chromosome 2 inactive DNA
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提交于 Apr 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"