概述

  • 产品名称Anti-HIC1抗体
    参阅全部 HIC1 一抗
  • 描述
    兔多克隆抗体to HIC1
  • 特异性This antibody is specific for HIC1.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A region within synthetic peptide: ALCASERRCS PLCGLDLSKK SPPGSAAPER PLAERELPPR PDSPPSAGPA, corresponding to amino acids 228-277 of Human HIC1

  • 阳性对照
    • Jurkat cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab49326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 74 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

靶标

  • 功能Transcriptional repressor. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall.
  • 组织特异性Ubiquitously expressed with highest levels found in lung, colon, prostate, thymus, testis and ovary. Expression is absent or decreased in many tumor cells.
  • 序列相似性Belongs to the krueppel C2H2-type zinc-finger protein family. Hic subfamily.
    Contains 1 BTB (POZ) domain.
    Contains 5 C2H2-type zinc fingers.
  • 翻译后修饰Acetylated on several residues, including Lys-333. Lys-333 is deacetylated by SIRT1.
    Sumoylated on Lys-333 by a PIAS family member, which positively regulates transcriptional repression ativity and is enhanced by HDAC4.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Hic 1 antibody
    • Hic-1 antibody
    • Hic1 antibody
    • HIC1_HUMAN antibody
    • Hypermethylated in cancer 1 antibody
    • Hypermethylated in cancer 1 protein antibody
    • ZBTB29 antibody
    • Zinc finger and BTB domain-containing protein 29 antibody
    • ZNF901 antibody
    see all

Anti-HIC1 antibody 图像

  • Lane 1 : MARKER
    Lane 2 : Anti-HIC1 antibody (ab49326) at 0.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 1 :
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 74 kDa
    Observed band size : 71 kDa (why is the actual band size different from the predicted?)

Anti-HIC1 antibody (ab49326)参考文献

ab49326 has not yet been referenced specifically in any publications.

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