概述

  • 产品名称
  • 描述
    兔多克隆抗体to HIC1
  • 特异性
    This antibody is specific for HIC1.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A region within synthetic peptide: ALCASERRCS PLCGLDLSKK SPPGSAAPER PLAERELPPR PDSPPSAGPA, corresponding to amino acids 228-277 of Human HIC1

  • 阳性对照
    • Jurkat cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab49326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 74 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

靶标

  • 功能
    Transcriptional repressor. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall.
  • 组织特异性
    Ubiquitously expressed with highest levels found in lung, colon, prostate, thymus, testis and ovary. Expression is absent or decreased in many tumor cells.
  • 序列相似性
    Belongs to the krueppel C2H2-type zinc-finger protein family. Hic subfamily.
    Contains 1 BTB (POZ) domain.
    Contains 5 C2H2-type zinc fingers.
  • 翻译后修饰
    Acetylated on several residues, including Lys-333. Lys-333 is deacetylated by SIRT1.
    Sumoylated on Lys-333 by a PIAS family member, which positively regulates transcriptional repression ativity and is enhanced by HDAC4.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Hic 1 antibody
    • HIC ZBTB transcriptional repressor 1 antibody
    • Hic-1 antibody
    • Hic1 antibody
    • HIC1_HUMAN antibody
    • Hypermethylated in cancer 1 antibody
    • Hypermethylated in cancer 1 protein antibody
    • ZBTB29 antibody
    • Zinc finger and BTB domain-containing protein 29 antibody
    • ZNF901 antibody
    see all

图片

  • Lane 1 : MARKER
    Lane 2 : Anti-HIC1 antibody (ab49326) at 0.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 1 :
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 74 kDa
    Observed band size : 71 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab49326 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (Fibroblasts)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
100000 cells
Specification
Fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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提交于 Mar 29 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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