Anti-hHR23A抗体[EPR4818] (ab108592)


  • 产品名称Anti-hHR23A抗体[EPR4818]
    参阅全部 hHR23A 一抗
  • 描述
    兔单克隆抗体[EPR4818] to hHR23A
  • 经测试应用适用于: WB, Flow Cytmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human hHR23A.

  • 阳性对照
    • MCF7, HeLa, Raji, and Jurkat cell lysates; Jurkat cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab108592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 40 kDa.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC,IHC-P or IP.
  • 靶标

    • 功能Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.
      Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC.
      Involved in vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 vpr with the host proteasome.
    • 序列相似性Belongs to the RAD23 family.
      Contains 2 UBA domains.
      Contains 1 ubiquitin-like domain.
    • 结构域The ubiquitin-like domain mediates interaction with ATXN3.
      The ubiquitin-like (UBL) and the UBA (ubiquitin-associated) domains interact intramolecularly in a highly dynamic manner, as each UBA domain competes for an overlapping UBL domain surface. Binding of ubiquitin or proteasome subunit PSMD4 disrupt the UBL-UBA domain interactions and drive RAD23A in to an open conformation.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • hHR 23A antibody
      • hHR23A antibody
      • HR23A antibody
      • MGC111083 antibody
      • RAD 23a antibody
      • RAD23 homolog A (S. cerevisiae) antibody
      • RAD23 homolog A antibody
      • RAD23 yeast homolog A antibody
      • RAD23A antibody
      • RD23A_HUMAN antibody
      • UV excision repair protein RAD23 antibody
      • UV excision repair protein RAD23 homolog A antibody
      see all

    Anti-hHR23A antibody [EPR4818] 图像

    • All lanes : Anti-hHR23A antibody [EPR4818] (ab108592) at 1/10000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 40 kDa
    • ab108592 at 1/10 dilution staining hHR23A in permeabilized Jurkat cells by Flow cytometry (shown in red). Rabbit IgG (negative) in green.

    Anti-hHR23A antibody [EPR4818] (ab108592)参考文献

    ab108592 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (u2os)
    Specification u2os
    Permeabilization No
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    提交于 Jan 02 2014

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (12%)
    Sample Human Cell lysate - whole cell (U2OS)
    Specification U2OS
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C

    Abcam user community

    Verified customer

    提交于 Jan 02 2014