Anti-Hho1抗体- ChIP Grade (ab71833)

概述

  • 产品名称Anti-Hho1抗体- ChIP Grade
  • 描述
    兔多克隆抗体to Hho1 - ChIP Grade
  • 特异性This antibody recognizes linker histone from S. Cerevisiae.
  • 经测试应用适用于: ChIP, CHIPseq, WBmore details
  • 种属反应性
    与反应: Saccharomyces cerevisiae
  • 免疫原

    Recombinant full length protein purified from E. coli.

性能

应用

Our Abpromise guarantee covers the use of ab71833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration. PubMed: 22689825
CHIPseq Use at an assay dependent concentration. PubMed: 22689825
WB 1/1000. Predicted molecular weight: 28 kDa.

靶标

  • 相关性Hho1 could act as an H1-type linker histone. It has been shown to bind DNA.
  • 细胞定位Nuclear
  • 数据库链接
    • 别名
      • Hho1p antibody
      • Histone H1, a linker histone required for nucleosome packaging at restricted sites antibody
      • LPI17C antibody
      • Not required for telomeric silencing, basal transcriptional repression, or efficient sporulation antibody
      • Suppresses DNA repair involving homologous recombination antibody
      • YPL127C antibody
      see all

    Anti-Hho1 antibody - ChIP Grade 图像



    • Predicted band size : 28 kDa
    • All lanes : Anti-Hho1 antibody - ChIP Grade (ab71833) at 1/1000 dilution

      Lane 1 : Wild type S. cerevisiae whole cell lysate
      Lane 2 : Hho1 knock-out S. cerevisiae whole cell lysate
      Lane 3 : S. cerevisiae whole cell lysate expressing Hho1 with tag

      Lysates/proteins at 15 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilution

      Predicted band size : 28 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)

      This image is courtesy of an anonymous Abreview

      See Abreview

    Anti-Hho1 antibody - ChIP Grade (ab71833)参考文献

    This product has been referenced in:
    • Brush GS Evidence that histone H1 is dispensable for proper meiotic recombination in budding yeast. BMC Res Notes 8:275 (2015). Read more (PubMed: 26122007) »
    • Sun Z  et al. VEGFR2 induces c-Src signaling and vascular permeability in vivo via the adaptor protein TSAd. J Exp Med 209:1363-77 (2012). WB, ChIP, CHIPseq ; Saccharomyces cerevisiae . Read more (PubMed: 22689825) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Saccharomyces cerevisiae Cell lysate - whole cell (cycling haploid cells)
    Loading amount 15 µg
    Specification cycling haploid cells
    Gel Running Conditions Reduced Denaturing (4-12%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 23 2013

    Thank you for contacting us.

    I have checked with the lab, butunfortunately, they are unable to provide the antibody in purified, glycerol-free form.

    I am sorry to not have more helpful information, but hope my recent email gave yo...

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    Thank you for your email.

    The antibody ab71833 comes as whole antiserum and is therefore not purified. The antibody concentration has not been determined as it would not be reflective of the concentration of the specific antibody in the seru...

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    I now have further information to share with the customer in regards to the protocol used to perform the western blotting experiment presented on the datasheet ofthe Anti-Hho1 antibody (ab71833).


    Theloading buffer used was a standard SD...

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    Thank you for contacting us.


    The Western blot presented on the datasheet ofAnti-Hho1 antibody (ab71833) wasperformed using extracts prepared by glass bead disruption into 20% TCA (the cell pellet was prepared from a 10 ml mid-log cultur...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"