概述

  • 产品名称Anti-Hhip抗体
    参阅全部 Hhip 一抗
  • 描述
    兔多克隆抗体to Hhip
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Hhip.

    (Peptide available as ab39947.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: HeLa Whole Cell; Mouse Heart; Mouse Liver; Mouse Pancreas.

性能

应用

Our Abpromise guarantee covers the use of ab39208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 79 kDa).

靶标

  • 功能Modulates hedgehog signaling in several cell types including brain and lung through direct interaction with members of the hedgehog family.
  • 组织特异性Widely expressed in fetal and adult tissues. Highest expression in adult heart, liver and pancreas, and in fetal kidney.
  • 序列相似性Belongs to the HHIP family.
    Contains 2 EGF-like domains.
  • 结构域A flexible loop interacts with the SHH zinc binding site and contributes to zinc binding.
  • 细胞定位Cytoplasm and Cell membrane. Secreted. The last 22 C-terminal amino acids may participate in cell membrane attachment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ20992 antibody
    • FLJ90230 antibody
    • Hedgehog interacting protein antibody
    • Hedgehog-interacting protein antibody
    • hedgehoginteracting protein antibody
    • HHIP antibody
    • HHIP_HUMAN antibody
    • Hip antibody
    see all

Anti-Hhip antibody 图像

  • All lanes : Anti-Hhip antibody (ab39208) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : Liver (Mouse) Tissue Lysate
    Lane 4 : Pancreas (Mouse) Tissue Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
    Lane 6 : Heart (Mouse) Tissue Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
    Lane 7 : Liver (Mouse) Tissue Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
    Lane 8 : Pancreas (Mouse) Tissue Lysate with Human Hhip peptide (ab39947) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 79 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,45 kDa,47 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

Anti-Hhip antibody (ab39208)参考文献

This product has been referenced in:
  • Wang C  et al. EZH2 Mediates epigenetic silencing of neuroblastoma suppressor genes CASZ1, CLU, RUNX3, and NGFR. Cancer Res 72:315-24 (2012). Read more (PubMed: 22068036) »
  • Zhou X  et al. Identification of a chronic obstructive pulmonary disease genetic determinant that regulates HHIP. Hum Mol Genet 21:1325-35 (2012). WB ; Human . Read more (PubMed: 22140090) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (various incl: HeLa Ms Liver Ms Kidney)
Loading amount 30 µg
Specification various incl: HeLa Ms Liver Ms Kidney
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Nov 14 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"