Anti-HGS (phospho Y334)抗体(ab62447)

概述

  • 产品名称Anti-HGS (phospho Y334)抗体
    参阅全部 HGS 一抗
  • 描述
    兔多克隆抗体to HGS (phospho Y334)
  • 特异性ab62447 detects endogenous levels of HGS only when phosphorylated at tyrosine 334.
  • 经测试应用适用于: ICC/IF, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human HGS around the phosphorylation site of tyrosine 334 (R-N-YP-W-E).

  • 阳性对照
    • IHC-P: Human brain tissue ICC/IF: HeLa cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab62447 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab62447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.
IHC-P 1/50 - 1/100.

靶标

  • 功能Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
  • 组织特异性Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.
  • 序列相似性Contains 1 FYVE-type zinc finger.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
    Contains 1 VHS domain.
  • 结构域Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.
    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • 翻译后修饰Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.
    Ubiquitinated by ITCH.
  • 细胞定位Cytoplasm. Early endosome membrane. Endosome > multivesicular body membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Hepatocyte growth factor regulated tyrosine kinase substrate antibody
    • Hepatocyte growth factor-regulated tyrosine kinase substrate antibody
    • HGF-regulated tyrosine kinase substrate antibody
    • HGNC:4897 antibody
    • HGS antibody
    • HGS_HUMAN antibody
    • Hrs antibody
    • Human growth factor regulated tyrosine kinase substrate antibody
    • Protein pp110 antibody
    • ZFYVE8 antibody
    see all

Anti-HGS (phospho Y334) antibody 图像

  • Immunohistochemistry analysis of HGS in human brain tissue (paraffin section) using ab62447 (1/50); in the presence or absence of phosphopeptide.
  • Immunofluorescence analysis of HGS in HeLa cells treated with Forskolin (40nM, 15mins); using ab62447 (1/500).

Anti-HGS (phospho Y334) antibody (ab62447)参考文献

ab62447 has not yet been referenced specifically in any publications.

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