概述

  • 产品名称Anti-HGS抗体
    参阅全部 HGS 一抗
  • 描述
    小鼠单克隆抗体to HGS
  • 经测试应用适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Recombinant fragment: KLEIMRQKKQ EYLEVQRQLA IQRLQEQEKE RQMRLEQQKQ TVQMRAQMPA FPLPYAQLQA MPAAGGVLYQ PSGPASFPST FSPAGSVEGS PMHGVYMSQP , corresponding to amino acids 513-612 of Human HGS

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal pancreas.

性能

应用

Our Abpromise guarantee covers the use of ab56468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 86 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/400. PMID 19684575
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
  • 组织特异性Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.
  • 序列相似性Contains 1 FYVE-type zinc finger.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
    Contains 1 VHS domain.
  • 结构域Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.
    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • 翻译后修饰Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.
    Ubiquitinated by ITCH.
  • 细胞定位Cytoplasm. Early endosome membrane. Endosome > multivesicular body membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Hepatocyte growth factor regulated tyrosine kinase substrate antibody
    • Hepatocyte growth factor-regulated tyrosine kinase substrate antibody
    • HGF-regulated tyrosine kinase substrate antibody
    • HGNC:4897 antibody
    • HGS antibody
    • HGS_HUMAN antibody
    • Hrs antibody
    • Human growth factor regulated tyrosine kinase substrate antibody
    • Protein pp110 antibody
    • ZFYVE8 antibody
    see all

Anti-HGS antibody 图像

  • IHC image of HGS staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab56468, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.



  • Predicted band size : 86 kDa
    HGS antibody (ab56468) at 1ug/lane + K-562 cell lysate at 25ug/lane.
  • Overlay histogram showing Jurkat cells stained with ab56468 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56468, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-HGS antibody (ab56468)参考文献

This product has been referenced in:
  • Kolb AR  et al. ESCRT regulates surface expression of the Kir2.1 potassium channel. Mol Biol Cell 25:276-89 (2014). Human . Read more (PubMed: 24227888) »
  • Stalder L  et al. The rough endoplasmatic reticulum is a central nucleation site of siRNA-mediated RNA silencing. EMBO J 32:1115-27 (2013). WB, ICC/IF ; Human . Read more (PubMed: 23511973) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Rat Cell lysate - whole cell (Neurons)
Specification Neurons
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Mar 12 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"